Impaired toll-like receptor signalling in peripheral B cells from newly diagnosed type-2 diabetic subjects.

Abstract

Toll-like receptors (TLRs) under diabetic conditions trigger inflammation and impair immunity. In the present study, we looked at the expression of TLRs (2 and 4) and their adaptors in Normal Glucose Tolerant (NGT), Newly Diagnosed Type-2 Diabetic (NDD) and Known Type-2 Diabetic (KDM) subjects. We also estimated TLR induced cytokine secretion, cellular activation and apoptosis. Surface expression of TLR2 and 4 was significantly reduced in the B cells of the NDD subjects and was associated with decreased cellular activation and cytokine secretion (TNF-α and IL-6). This impairment was not due to B cell deficiency or apoptosis or immunosuppressive cytokine (IL-10 and TGF-β) secretion. However, the upregulation of immunomodulatory enzymes (Arg-1, HO-1 and IDO) could probably account for the reduced TLR expression. The defective TLR signalling was largely ameliorated in the KDM group which might be due to the use the anti-diabetic drugs which have anti-inflammatory effect.

• Authors: Haridoss Madhumitha, Viswanathan Mohan, Nathella P Kumar, Rajendra Pradeepa, Subash Babu, Vivekanandhan Aravindhan
• Journal: Cytokine (Cytokine) Vol. 76 Issue 2 Pg. 253-259 (Dec 2015) ISSN: 1096-0023 [Electronic] England
• PMID: 25959254 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Antigens, CD
• Antigens, Differentiation, T-Lymphocyte
• CD69 antigen
• IDO1 protein, human
• IL10 protein, human
• Indoleamine-Pyrrole 2,3,-Dioxygenase
• Interleukin-6
• Lectins, C-Type
• TNF protein, human
• Toll-Like Receptor 2
• Toll-Like Receptor 4
• Transforming Growth Factor beta
• Tumor Necrosis Factor-alpha
• Interleukin-10

Topics

• Adult
• Antigens, CD (genetics)
• Antigens, Differentiation, T-Lymphocyte (genetics)
• Apoptosis
• B-Lymphocytes (immunology, metabolism, pathology)
• Cells, Cultured
• Diabetes Mellitus (diagnosis, immunology)
• Female
• Humans
• Indoleamine-Pyrrole 2,3,-Dioxygenase (genetics)
• Interleukin-10 (genetics)
• Interleukin-6 (genetics)
• Lectins, C-Type (genetics)
• Male
• Middle Aged
• Signal Transduction
• Toll-Like Receptor 2 (genetics, immunology, metabolism)
• Toll-Like Receptor 4 (genetics, immunology, metabolism)
• Transforming Growth Factor beta (genetics)
• Tumor Necrosis Factor-alpha (genetics)