[Effect of Storage Time on Cumulation of Platelet-related Growth Factors in the Supernatant of Leukoreduced Packed Red Cells and Tumor Cell Proliferation In Vitro].

Abstract

OBJECTIVE:

To investigate the effect of storage time on accumulation of platelet-related growth factors in the supernatant of leukoreduced packed red blood cells (LR-pRBC) and on tumor cell proliferation in vitro.

METHODS:

LR-pRBC were quartered and stored at 2 °C-6 °C. The supernatant of pRBC was obtained by centrifugation with 1 006 × g for 10 min at day 0, 14, 21 and 35 d. The enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of platelet-derived growth factor(PDGF) and vascular endothelial growth factor (VEGF). After HepG2 cells was cultured with the supernatant of LR pRBC at day 0 and day 35 together for 48 hours, methylthiazoliltetracolium (MTT) method was used to measure the proliferation of tumor cells in vitro.

RESULTS:

The concentrations of 2 cytokines were still increased with the storage time prolonging. As compared to LR-pRBC at day 0 [611.84 (95%CI, 356.45-867.23) pg/ml], the level of VEGF reached 900.16 (95% CI, 552.26-1248.07) pg/ml (P<0.05). There was a similar tendency in PDGF level with less increment in the supernatant of LR pRBC at day 35 [2.23 (95% CI, 0-5.37) ng/L vs 5.66 (95% CI, 0-12.48), P=0.073], but there was no significant statistical difference. Likewise, in vitro study of HepG2 cell proliferation showed that the LR-pRBC at day 35 promoted more proliferation of tumor cells with OD value 0.40 (95% CI, 0.38-0.42) (P<0.05).

CONCLUSION:

The residual platelets in LR-pRBC were activated, disintegrated and released the dense granules and α-granules which induce the accumulation of VEGF and PDGF. It seemed that the supernatant of LR-pRBC promoted the proliferation of tumor cells in vitro.

• Authors: Yuan Zhuang, Ting Zhang, Chao Wei, Ji-Chun Pan, Shu-Fang Wang, Ai-Qun Zhang, De-Qing Wang
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 23 Issue 2 Pg. 541-5 (Apr 2015) ISSN: 1009-2137 [Print] China
• PMID: 25948221 (Publication Type: English Abstract, Journal Article)

Chemical References

• Cytokines
• Platelet-Derived Growth Factor
• VEGFA protein, human
• Vascular Endothelial Growth Factor A

Topics

• Blood Platelets
• Blood Preservation
• Cell Proliferation
• Cytokines
• Enzyme-Linked Immunosorbent Assay
• Humans
• In Vitro Techniques
• Platelet-Derived Growth Factor
• Time Factors
• Vascular Endothelial Growth Factor A