Abstract | OBJECTIVE: METHODS AND RESULTS: We initially identified plasma miR-122, -140-3p, -144, -720, -1225-3p, -2861, and -3149 as candidate miRNAs associated with AMI (≥2 fold and P < 0.05) by comparing expression differences of miRNAs among AMI, non- coronary heart disease (non-CHD) and stable angina (SA) groups, using miRNA microarrays (n = 8 independent arrays in each group). Those seven plasma miRNAs were further examined with qRT-PCR analyses in two replications including 111 and 428 patients separately, and the results demonstrated that plasma miR-122, -140-3p, -720, -2861, and -3149 were elevated in the ACS group vs. the non-ACS (non-CHD + SA) group (P < 0.01). The area under the receiver operating characteristic curve (AUC) of the five miRNAs for ACS classification was 0.838, 0.818, 0.865, 0.852, and 0.670, respectively (all P < 0.001), while the values reached 0.843 and 0.925 when simultaneously with miR-122 and -3149 or with miR-122, -2861, and -3149 together (all P < 0.001). In plasma of pigs after coronary ligation, miR-122 was increased from 180 min to 240 min and miR-3149 was augmented from 30 min to 240 min compared with the sham pigs (all P < 0.05). CONCLUSION: Plasma miR-122, -140-3p, -720, -2861, and -3149 were associated with and potentially novel biomarkers for ACS.
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Authors | Xiangdong Li, Yuejin Yang, Laiyuan Wang, Shubin Qiao, Xiangfeng Lu, Yongjian Wu, Bo Xu, Hongfan Li, Dongfeng Gu |
Journal | PloS one
(PLoS One)
Vol. 10
Issue 5
Pg. e0125430
( 2015)
ISSN: 1932-6203 [Electronic] United States |
PMID | 25933289
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- MIRN122 microRNA, human
- MIRN2861 microRNA, human
- MIRN3149 microRNA, human
- MIRN720 microRNA, human
- MicroRNAs
- Mirn140 microRNA, human
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Topics |
- Acute Coronary Syndrome
(blood, diagnosis, genetics)
- Angina, Unstable
(blood, diagnosis, genetics)
- Animals
- Area Under Curve
- Biomarkers
(blood)
- Gene Expression Profiling
- Gene Expression Regulation
- Humans
- Male
- MicroRNAs
(blood, genetics)
- Myocardial Infarction
(blood, diagnosis, genetics)
- Oligonucleotide Array Sequence Analysis
- ROC Curve
- Swine
- Swine, Miniature
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