Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells.

Abstract

Melia azedarach (MA) has been used in folk medicine in Asia for the treatment of several diseases. Several constituents from MA possess anti-herpetic, anti-angiogenic and anticancer properties. The aim of the present study was to investigate the effect of a 70% ethanol extract of MA on melanogenesis and the underlying mechanisms involved. A B16F10 mouse melanoma cell line was used in our experiments. Treatment of B16F10 cells with the MA extract (10, 20 and 40 µg/ml) increased melanin content in a concentration-dependent manner without cytotoxicity at 24 h. Further experiments indicated that the MA extract (20 µg/ml) increased melanin content as early as at 4 h after treatment. Additionally, although the MA extract did not affect intracellular tyrosinase activity and the protein levels of tyrosinase and tyrosinase-related protein-2 (TRP-2) at 2 and 4 h after treatment, the MA extract increased TRP-1 protein expression at both time points. However, no significant effect of the MA extract treatment on TRP-1 mRNA level at the time points measured was observed. In conclusion, the results from the present study demonstrate that the MA extract increases melanogenesis through the upregulation of TRP-1 protein expression by post-transcriptional control in B16F10 cells and suggest that the MA extract can be viewed as a rapid inducer of melanogenesis, thus rendering it a potential treatment for hypopigmentation diseases including vitiligo.

• Authors: Cheng Yao, Cheng Long Jin, Inn Gyung Oh, Chi-Hyun Park, Jin Ho Chung
• Journal: International journal of molecular medicine (Int J Mol Med) Vol. 35 Issue 6 Pg. 1761-6 (Jun 2015) ISSN: 1791-244X [Electronic] Greece
• PMID: 25872655 (Publication Type: Journal Article)

Chemical References

• Melanins
• Membrane Glycoproteins
• Neoplasm Proteins
• Plant Extracts
• Oxidoreductases
• Tyrp1 protein, mouse

Topics

• Animals
• Azadirachta (chemistry)
• Cell Line, Tumor
• Gene Expression Regulation, Neoplastic (drug effects)
• Melanins (biosynthesis)
• Melanoma (metabolism, pathology)
• Membrane Glycoproteins (biosynthesis)
• Mice
• Neoplasm Proteins (biosynthesis)
• Oxidoreductases (biosynthesis)
• Plant Extracts (chemistry, pharmacology)