Effect of carvedilol against myocardial injury due to ischemia-reperfusion of the brain in rats.

Abstract

We have previously reported the mechanism behind the myocardial injury and the activation of autonomic nervous system during the ischemia-reperfusion (IR) of the rat brain. This study was planned to investigate the effect of carvedilol, a β-blocker, in improving the myocardial injury caused by IR of the rat brain. We have used a whole cerebral IR model in rats by clamping both the right and left common carotid arteries. Rats were divided into five groups; Sham surgery group (Group-Sham), carvedilol treatment before ischemia group (Group-Is+C), vehicle control group (Group-Is+V), carvedilol treatment before reperfusion group (Group-Re+C) and the vehicle control group (Group-Re+V). We have measured the blood pressure and heart rate via a catheter, myocardial tissue β1-adrenaline receptor (β1-AR) levels, phosphor-p38 mitogen-activated protein kinase (p-p38 MAPK) signaling factor, malondialdehyde (MDA), and apoptosis (TUNEL assay and expression of caspase-7 protein). The results indicated that the increased expressions of β1-AR, p-p38 MAPK, caspase-7, apoptotic cells and MDA level in the myocardial tissue due to brain ischemia-reperfusion were significantly reduced by carvedilol treatment. From these observations we can suggest that, with the advantage of its antioxidant and β blocking action, carvedilol had played the improvement of myocardial injury in ischemia-reperfusion of the brain.

• Authors: Meilei Harima, Somasundaram Arumugam, Juan Wen, Vigneshwaran Pitchaimani, Vengadeshprabhu Karuppagounder, Mst Rejina Afrin, Remya Sreedhar, Shizuka Miyashita, Mayumi Nomoto, Kazuyuki Ueno, Masahiko Nakamura, Kenichi Watanabe
• Journal: Experimental and molecular pathology (Exp Mol Pathol) Vol. 98 Issue 3 Pg. 558-62 (Jun 2015) ISSN: 1096-0945 [Electronic] Netherlands
• PMID: 25872160 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Adrenergic beta-Antagonists
• Carbazoles
• Propanolamines
• Receptors, Adrenergic, beta-1
• Carvedilol
• Malondialdehyde
• p38 Mitogen-Activated Protein Kinases

Topics

• Adrenergic beta-Antagonists (therapeutic use)
• Animals
• Apoptosis
• Blood Pressure
• Brain (blood supply)
• Carbazoles (therapeutic use)
• Carvedilol
• Heart (drug effects, physiopathology)
• Heart Rate
• Male
• Malondialdehyde (metabolism)
• Myocardium (metabolism, pathology)
• Propanolamines (therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Receptors, Adrenergic, beta-1 (genetics, metabolism)
• Reperfusion Injury (drug therapy, physiopathology)
• p38 Mitogen-Activated Protein Kinases (genetics, metabolism)