Protective Effect of Hydroxytyrosol Against Cardiac Remodeling After Isoproterenol-Induced Myocardial Infarction in Rat.

Abstract

The present study aimed to investigate the cardioprotective effect of hydroxytyrosol (HT) against isoproterenol-induced myocardial infarction in rats. Male rats were randomly divided into four groups, control, isoproterenol (Isop) and pretreated animals with HT in two different doses (2 and 5 mg/kg) orally for 7 days and intoxicated with isoproterenol (Isop + HT1) and (Isop + HT2) groups. Myocardial infarction in rats was induced subcutaneously by isoproterenol (100 mg/kg, s.c.) at an interval of 24 h on 6th and 7th day. On 8th day, electrocardiographic (ECG) pattern, gravimetric and biochemical parameters were assessed. Isoproterenol exhibited changes in ECG pattern, including significant ST-segment elevation and increase in the serum troponin-T level by 317 % as compared to control rats. Moreover, cardiac injury markers (creatine kinase-MB, lactate dehydrogenase, alanine aminotransferase) underwent a notable rise in serum of infarcted animals. Else, a disturbance in lipids profile and significant increase in lipase and angiotensin-converting enzyme (ACE) activities and heart weight ratio were observed in isoproterenol group. However, pre- and co-treatment with HT (2 and 5 mg/kg) improved the myocardium injury, restored the hemodynamic function and inhibited the ACE activity that prevent cardiac hypertrophy and remodeling. Overall, these findings demonstrated that HT exerted a potent cardioprotective effect against isoproterenol-induced myocardial infarction.

• Authors: Kais Mnafgui, Raouf Hajji, Fatma Derbali, Ines Khlif, Ftouma Kraiem, Hedi Ellefi, Abdelfattah Elfeki, Noureddine Allouche, Neji Gharsallah
• Journal: Cardiovascular toxicology (Cardiovasc Toxicol) Vol. 16 Issue 2 Pg. 147-55 (Apr 2016) ISSN: 1559-0259 [Electronic] United States
• PMID: 25846342 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cardiotonic Agents
• 3,4-dihydroxyphenylethanol
• Isoproterenol
• Phenylethyl Alcohol

Topics

• Animals
• Cardiotonic Agents (pharmacology, therapeutic use)
• Isoproterenol (toxicity)
• Male
• Myocardial Infarction (chemically induced, pathology, prevention & control)
• Phenylethyl Alcohol (analogs & derivatives, pharmacology, therapeutic use)
• Rats
• Rats, Wistar
• Ventricular Remodeling (drug effects, physiology)