Abstract | BACKGROUND: OBJECTIVE: To determine if psoriasis is associated with pervasive expression of antiviral genes in skin and blood. METHODS: We performed RNA sequencing on skin samples of 18 subjects with chronic plaque psoriasis and 16 healthy controls. We examined the expression of a predefined set of 42 antiviral genes, each of which has been shown in previous studies to inhibit viral replication. In parallel, we examined antiviral gene expression in atopic dermatitis, non-lesional psoriatic skin and psoriatic blood. We performed HIV-1 infectivity assays in CD4+ peripheral blood T cells from psoriatic and healthy individuals. RESULTS: We observed significant overexpression of 16 antiviral genes in lesional psoriatic skin, with a greater than two-fold increase in ISG15, RSAD2, IRF7, MX2 and TRIM22 (P < 1E-07). None of these genes was overexpressed in atopic dermatitis skin (P < 0.0001) or non-lesional psoriatic skin. In contrast to the skin compartment, no differences in antiviral gene expression were detected in the peripheral blood of psoriasis cases compared to healthy controls. CD4+ T cells from both psoriatic and healthy patients supported HIV-1 infection at a similar rate. CONCLUSION: Our findings highlight psoriasis as an inflammatory disease with cutaneous but not systemic immune activation against viral pathogens.
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Authors | R A Raposo, R Gupta, M Abdel-Mohsen, M Dimon, M Debbaneh, W Jiang, V A York, K S Leadabrand, G Brown, M Malakouti, S Arron, P J Kuebler, J J Wu, S K Pillai, D F Nixon, W Liao |
Journal | Journal of the European Academy of Dermatology and Venereology : JEADV
(J Eur Acad Dermatol Venereol)
Vol. 29
Issue 10
Pg. 1951-7
(Oct 2015)
ISSN: 1468-3083 [Electronic] England |
PMID | 25809693
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 European Academy of Dermatology and Venereology. |
Chemical References |
- Cytokines
- IRF7 protein, human
- Interferon Regulatory Factor-7
- MX2 protein, human
- Minor Histocompatibility Antigens
- Myxovirus Resistance Proteins
- Proteins
- Repressor Proteins
- TRIM22 protein, human
- Tripartite Motif Proteins
- Ubiquitins
- ISG15 protein, human
- RNA
- Oxidoreductases Acting on CH-CH Group Donors
- RSAD2 protein, human
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Topics |
- Adult
- CD4-Positive T-Lymphocytes
(virology)
- Case-Control Studies
- Cells, Cultured
- Cytokines
(genetics)
- Dermatitis, Atopic
(genetics, immunology)
- Gene Expression
- Gene Expression Profiling
- HIV Infections
(genetics)
- Humans
- Interferon Regulatory Factor-7
(genetics)
- Minor Histocompatibility Antigens
- Myxovirus Resistance Proteins
(genetics)
- Oligonucleotide Array Sequence Analysis
- Oxidoreductases Acting on CH-CH Group Donors
- Proteins
(genetics)
- Psoriasis
(genetics, immunology, virology)
- RNA
(blood, metabolism)
- Repressor Proteins
(genetics)
- Skin
(immunology, metabolism)
- Tripartite Motif Proteins
- Ubiquitins
(genetics)
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