Abstract | OBJECTIVE: METHODS: Sixty adult male Wistar rats were randomly divided into five groups (n = 12): sham operation group ( sham), diabetes + sham operation group (DM + sham), diabetes + MI group (DM + MI) , diabetes + myocardial infarction + cobalt original porphyrin (CoPP) group (DM + MI + CoPP), diabetes + myocardial infarction + CoPP+ tin porphyrin (SnMP) group (DM + MI + CoPP + SnMP). CoPP 4.5 mg/kg or SnMP 15 mg/kg were administered at the day next to MI operation, for six weeks, once a week. At the 28th week post operation, the echocardiography, left heart via the carotid artery indoor intubation were used to observe the long-term influence of HO-1 inducer ( cobalt protoporphyrin, CoPP) and activity of HO inhibitor ( tin porphyrin, SnMP) on the indices of left ventricular remodeling and cardiac function after the intervention. Blood glucose (GLU), total cholesterol (TC), C-reactive protein (CRP), serum creatinine (Cr), aminotransferase (ALT) and other indicators were measured. ELISA was used to test interleukin-6 (IL-6), tumor necrosis factor (TNF), nitric oxide (NO), prostacyclin (PGI2), adiponectin, and ultra sensitive CRP ( HsCRP) level. RESULTS: HO-1 inducer, CoPP, could ameliorate ± dp/dtmax, left ventricular ejection fraction and left ventricular shortening fraction in diabetic myocardial infarction rats. It could also decrease left ventricular end-diastolic diameter. The serum bilirubin, NO and PGI2 levels, myocardial phosphorylated endothelial nitric oxide synthasee(peNOS), phosphorylated activated protein kinase (pAkt), phosphorylated adenosine monophosphate-activated protein kinase (pAMPK) expression were also significantly elevated, and the serum hs-CRP and TNF levels were significantly inhibited. Compared to inducer group, cardiac function were worse in the inhibitor group. CONCLUSION: Upregulated HO-1 level can improve the endothelial function, inhibite of the inflammatory response and enhance the antioxidant substances in serum bilirubin via peNOS-pAMPK pathway, which effectively inhibit ventricular remodeling and improve the long-term cardiac function after infarction in diabetic rats.
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Authors | Jian Li, Tian-Meng Chen, Jianla Cao, De-You Chen, Hao Wang, Hai-Yan Shi, Meng Gao, Fan Li, Nader G Abraham |
Journal | Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
(Zhongguo Ying Yong Sheng Li Xue Za Zhi)
Vol. 30
Issue 5
Pg. 421-6
(Sep 2014)
ISSN: 1000-6834 [Print] China |
PMID | 25571633
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Heme Oxygenase (Decyclizing)
- Hmox1 protein, rat
- Bilirubin
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Topics |
- Animals
- Antioxidants
(metabolism)
- Bilirubin
(blood)
- Diabetes Mellitus, Experimental
(physiopathology)
- Heme Oxygenase (Decyclizing)
(metabolism)
- Male
- Myocardial Infarction
(enzymology)
- Myocardium
(enzymology)
- Rats
- Rats, Wistar
- Signal Transduction
- Up-Regulation
- Ventricular Function, Left
- Ventricular Remodeling
(drug effects)
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