Abstract | BACKGROUND: METHODS: RESULTS: Intratracheal administration of quercetin decreased the wet lung-to- body weight ratio. Moreover, quercetin decreased MMP-9 activity and the production of pro-inflammatory cytokines in BALF cells activated by LPS in advance. We determined the expression of quercetin-induced HO-1 in mouse lung, e.g., alveolar macrophages (AMs), alveolar and bronchial epithelial cells. When AMJ2-C11 cells were cultured with quercetin, a marked suppression of LPS-induced pro-inflammatory cytokine production was observed. The cytoprotective effects were attenuated by the addition of the HO-1 inhibitor SnPP. These results indicated that quercetin suppressed LPS-induced lung inflammation, and that an HO-1-dependent pathway mediated these cytoprotective effects. CONCLUSIONS: Our findings indicated that quercetin suppressed LPS-induced lung inflammation, and that an HO-1-dependent pathway mediated these cytoprotective effects. Intratracheal administration of quercetin will lead to new supportive strategies for cytoprotection in these serious lung conditions.
|
Authors | Koji Takashima, Miyoko Matsushima, Katsunori Hashimoto, Haruka Nose, Mitsuo Sato, Naozumi Hashimoto, Yoshinori Hasegawa, Tsutomu Kawabe |
Journal | Respiratory research
(Respir Res)
Vol. 15
Pg. 150
(Nov 21 2014)
ISSN: 1465-993X [Electronic] England |
PMID | 25413579
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Anti-Inflammatory Agents
- IL1B protein, mouse
- Inflammation Mediators
- Interleukin-1beta
- Interleukin-6
- Lipopolysaccharides
- Membrane Proteins
- Tumor Necrosis Factor-alpha
- interleukin-6, mouse
- Quercetin
- Heme Oxygenase-1
- Hmox1 protein, mouse
- Matrix Metalloproteinase 9
- Mmp9 protein, mouse
|
Topics |
- Acute Lung Injury
(chemically induced, enzymology, immunology, pathology, prevention & control)
- Administration, Inhalation
- Animals
- Anti-Inflammatory Agents
(administration & dosage)
- Bronchoalveolar Lavage Fluid
(chemistry, immunology)
- Cell Line
- Cytoprotection
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Heme Oxygenase-1
(metabolism)
- Inflammation Mediators
(metabolism)
- Interleukin-1beta
(metabolism)
- Interleukin-6
(metabolism)
- Intubation, Intratracheal
- Lipopolysaccharides
- Lung
(drug effects, enzymology, immunology, pathology)
- Macrophages, Alveolar
(drug effects, enzymology, immunology)
- Matrix Metalloproteinase 9
(metabolism)
- Membrane Proteins
(metabolism)
- Mice, Inbred C57BL
- Quercetin
(administration & dosage)
- Signal Transduction
(drug effects)
- Tumor Necrosis Factor-alpha
(metabolism)
|