Abstract | AIM: METHODS: Thirty-four Balb/c mice were randomly assigned to five groups, including the control, CAC control, CAC + caffeine, colitis control and colitis + caffeine. Three animals were sacrificed every two weeks for blinded macroscopic inspection, histological analysis, and total RNA extraction. An immunofluorescent assay was performed using specimens from the colitis control and colitis + caffeine groups to investigate whether the protective effect of caffeine was associated with less oxidative DNA damage. In vitro, HT29 cells pre-stimulated with different concentrations of recombinant CHI3L1 protein and H2O2 were loaded with the DCFH-DA fluorescent probe to determine the effect of CHI3L1 on intracellular reactive oxygen species production. RESULTS: CONCLUSION:
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Authors | Jia-Yi Ma, Run-Hua Li, Kun Huang, Gao Tan, Chen Li, Fa-Chao Zhi |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 20
Issue 42
Pg. 15736-44
(Nov 14 2014)
ISSN: 2219-2840 [Electronic] United States |
PMID | 25400457
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adipokines
- Anti-Inflammatory Agents
- Anticarcinogenic Agents
- CHI3L1 protein, human
- Chil1 protein, mouse
- Chitinase-3-Like Protein 1
- Glycoproteins
- Lectins
- RNA, Messenger
- Caffeine
- Dextran Sulfate
- Hydrogen Peroxide
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Topics |
- Adipokines
(genetics, metabolism)
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Anticarcinogenic Agents
(pharmacology)
- Caffeine
(pharmacology)
- Chitinase-3-Like Protein 1
- Colitis
(chemically induced, complications, genetics, metabolism, pathology, prevention & control)
- Colon
(drug effects, metabolism, pathology)
- Colonic Neoplasms
(etiology, genetics, metabolism, pathology, prevention & control)
- DNA Damage
- Dextran Sulfate
- Glycoproteins
(genetics, metabolism)
- HT29 Cells
- Humans
- Hydrogen Peroxide
(pharmacology)
- Lectins
(genetics, metabolism)
- Male
- Mice, Inbred BALB C
- Oxidative Stress
(drug effects)
- RNA, Messenger
(metabolism)
- Up-Regulation
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