Abstract | OBJECTIVE: MATERIALS AND METHODS: Commercial RCC tissue microarray arrays and a kidney cancer quantitative polymerase chain reaction array were used to examine Anxa2 by immunohistochemistry and real-time polymerase chain reaction analysis. Short hairpin (sh) RNA-based lentiviral system technology was used to evaluate the effects of manipulating Anxa2 expression on multiple malignant features of 2 RCC cell lines, A498 and 786-O, and its mechanisms. RESULTS: (1) The Anxa2 expression level was generally elevated to varying degrees in RCC tissues. In adjacent noncancerous tissues, Anxa2 was mainly expressed in glomeruli and slightly expressed in the cytoplasm of proximal tubules. (2) An increased Anxa2 expression level was found in tissues of clear cell RCC, papillary RCC, and chromophobe RCC, and it was prominently expressed in cancer cell membranes. In addition, the Anxa2 expression level was correlated with poor prognosis. (3) Silencing Anxa2 expression suppressed the abilities of cell migration and invasion, but cell proliferation was less affected. (4) Diminished Anxa2 expression caused alterations in the cell polarity, disrupted the formation of actin filaments, and reduced CXCR4 expression. (5) Inhibition of the Rho/Rock axis restored silencing of Anxa2-mediated suppression of cell motility. CONCLUSIONS: Overall, our study points out the regulatory function of Anxa2 in RCC cell motility and provides a molecular-based mechanism of Anxa2 positivity in the progression of RCC.
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Authors | Shun-Fa Yang, Han-Lin Hsu, Tai-Kuang Chao, Chia-Jung Hsiao, Yung-Feng Lin, Chao-Wen Cheng |
Journal | Urologic oncology
(Urol Oncol)
Vol. 33
Issue 1
Pg. 22.e11-22.e21
(Jan 2015)
ISSN: 1873-2496 [Electronic] United States |
PMID | 25284003
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Inc. All rights reserved. |
Chemical References |
- ANXA2 protein, human
- Actins
- Annexin A2
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Topics |
- Actin Cytoskeleton
(metabolism)
- Actins
(biosynthesis)
- Annexin A2
(biosynthesis, genetics)
- Carcinoma, Renal Cell
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
(physiology)
- Cell Polarity
(physiology)
- Cell Proliferation
(physiology)
- Female
- Humans
- Kidney Neoplasms
(genetics, metabolism, pathology)
- Male
- Neoplasm Invasiveness
- Prognosis
- Up-Regulation
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