Abstract | AIM: METHODS: Human FANCA lymphoblasts GM13022 and HCS536 were challenged with NM at concentration range within 10-1000 µg/ml. Cell toxicity was assessed by Trypan blue dye exclusion test. Invasion was evaluated through Matrigel and gelatinase zymography for MMP activity. RESULTS: NM was toxic in dose dependent mode to HCS536 cells but not to GM13022 cells. GM13022 cells but not HCS536 cells exhibited MMP-9 secretion, which was inhibited by NM. Matrigel invasion was inhibited in HCS536 cells at 100 and 500 µg/ml by 27% and 93%, respectively. In GM13022 cells, the NM showed completely blocked Matrigel invasion at 500 µg/ml. CONCLUSION: NM inhibited MMP secretion and Matrigel invasion in FANCA and inhibited invasion and induced toxicity in FANCC lymphoblasts. These results suggest that the NM may have therapeutic potential in Fanconi anemia associated neoplasia.
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Authors | M W Roomi, B Bhanap, N W Roomi, A Niedzwiecki, M Rath |
Journal | Experimental oncology
(Exp Oncol)
Vol. 36
Issue 3
Pg. 212-4
(Sep 2014)
ISSN: 2312-8852 [Electronic] Ukraine |
PMID | 25265357
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- FANCA protein, human
- FANCC protein, human
- Fanconi Anemia Complementation Group A Protein
- Fanconi Anemia Complementation Group C Protein
- Plant Extracts
- Tea
- Proline
- Matrix Metalloproteinases
- Lysine
- Ascorbic Acid
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Topics |
- Ascorbic Acid
(administration & dosage)
- Blotting, Western
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Fanconi Anemia
(drug therapy, metabolism, pathology)
- Fanconi Anemia Complementation Group A Protein
(metabolism)
- Fanconi Anemia Complementation Group C Protein
(metabolism)
- Humans
- In Vitro Techniques
- Lymphocytes
(drug effects, metabolism, pathology)
- Lysine
(administration & dosage)
- Matrix Metalloproteinases
(chemistry, metabolism)
- Plant Extracts
(pharmacology)
- Proline
(administration & dosage)
- Tea
(chemistry)
- Tumor Cells, Cultured
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