Inhibition of microtubule dynamics affects podosome belt formation during osteoclast induction.

Abstract

Osteoclast is the only cell that can degrade bone tissue in vivo. Recent studies have shown the important role of cytoskeleton dynamics in osteolysis and the formation of podosome belt in osteoclasts. This process is regulated by the dynamic microtubule (MT) network. We treated osteoclast precursor cells Raw264.7 with low concentration of nocodazole (10 nM) and antineoplastic drug taxol (10 nM) to block MT turnover, and used end binding protein 1 fused to GFP to track the movement of microtubules in induced osteoclasts. We show that low concentrations of nocodazole and taxol interfere with the formation of podosome belt, and reduce TRAP activity of induced osteoclasts. These results suggest that the effect of taxol on MT dynamics may be used clinically to reduce osteoclast activity and potentially prevent development of osteoporosis and other metabolic bone diseases.

• Authors: Yunfan Ti, Lingjun Zhou, Rui Wang, Jianning Zhao
• Journal: Cell biochemistry and biophysics (Cell Biochem Biophys) Vol. 71 Issue 2 Pg. 741-7 (Mar 2015) ISSN: 1559-0283 [Electronic] United States
• PMID: 25257130 (Publication Type: Journal Article)

Chemical References

• EB1 protein, mouse
• Isoenzymes
• Microtubule-Associated Proteins
• RANK Ligand
• Acid Phosphatase
• Acp5 protein, mouse
• Tartrate-Resistant Acid Phosphatase
• Paclitaxel
• Nocodazole

Topics

• Acid Phosphatase (metabolism)
• Animals
• Cell Count
• Cell Differentiation (drug effects)
• Cell Line
• Cell Membrane (drug effects, metabolism)
• Dose-Response Relationship, Drug
• Isoenzymes (metabolism)
• Mice
• Microtubule-Associated Proteins (metabolism)
• Microtubules (drug effects, metabolism)
• Nocodazole (pharmacology)
• Osteoclasts (cytology, drug effects, metabolism)
• Paclitaxel (pharmacology)
• RANK Ligand (pharmacology)
• Tartrate-Resistant Acid Phosphatase