Anti-epileptic effects of neuropeptide Y gene transfection into the rat brain.

Abstract

Neuropeptide Y gene transfection into normal rat brain tissue can provide gene overexpression, which can attenuate the severity of kainic acid-induced seizures. In this study, a recombinant adeno-associated virus carrying the neuropeptide Y gene was transfected into brain tissue of rats with kainic acid-induced epilepsy through stereotactic methods. Following these transfections, we verified overexpression of the neuropeptide Y gene in the epileptic brain. Electroencephalograms showed that seizure severity was significantly inhibited and seizure latency was significantly prolonged up to 4 weeks after gene transfection. Moreover, quantitative fluorescent PCR and western blot assays revealed that the mRNA and protein expression of the N-methyl-D-aspartate receptor subunits NR1, NR2A, and NR2B was inhibited in the hippocampus of epileptic rats. These findings indicate that neuropeptide Y may inhibit seizures via down-regulation of the functional expression of N-methyl-D-aspartate receptors.

• Authors: Changzheng Dong, Wenqing Zhao, Wenling Li, Peiyuan Lv, Xiufang Dong
• Journal: Neural regeneration research (Neural Regen Res) Vol. 8 Issue 14 Pg. 1307-15 (May 15 2013) ISSN: 1673-5374 [Print] India
• PMID: 25206425 (Publication Type: Journal Article)