Glioblastoma tumor cells release microvesicles, which contain
mRNA,
miRNA and
angiogenic proteins. These
tumor-derived microvesicles transfer genetic information and
proteins to normal cells. Previous reports demonstrated that the increased microvesicles in cerebrospinal fluid (CSF) of patients with
glioblastoma up-regulate procoagulant activity. The concentration of microvesicles was closely related to
thromboembolism incidence and clinical
therapeutic effects of
glioblastoma patients. However, it is still not clear how CSF microvesicles and what factors affect
glioblastoma development. In this study, we collected the plasma and CSF from
glioblastoma patients and healthy volunteers. Microvesicles acquired from serum or CSF were added to cultured endothelial cells. And the effects of these microvesicles on endothelial cells were examined. Our results showed that microvesicles from CSF of patients, but not from circulating blood, promoted endothelial cells migration and proliferation in vitro. In addition, the degree of endothelial cell proliferation triggered by microvesicles from CSF was reduced when treated with
siRNA targeting Akt/
beta-catenin, suggesting that the Akt/
beta-catenin pathway is involved in the microvesicle-initiated endothelial cell proliferation. In conclusion,
glioblastoma mainly affects microvesicles within CSF without showing significant impact on microvesicles in circulating blood. Microvesicles from the CSF of
glioblastoma patients may initiate endothelial cell growth and thus promote cell invasion. This effect may be directly exerted by activated Akt/
beta-catenin pathway.