Abstract | AIMS:
Sodium nitrite, a preservative used in meat products, helps in the production of free radicals, leading to increased lipid peroxidation, which plays a vital role in posing toxic effects in different body organs. On the other hand, arjunolic acid possesses antioxidant properties and plays protective roles against chemically induced organ pathophysiology. We investigated the effect of sodium nitrite on cardiac tissue in rats on the inflammatory cytokine balance and the type of induced apoptosis, and we analyzed the protective role of arjunolic acid. MAIN METHODS: Sixty adult male Sprague-Dawley rats were injected with 80mg/kg sodium nitrite in the presence/absence of arjunolic acid (100 and 200mg/kg). Cardiac pro-inflammatory cytokines (TNF-α and IL-1β), c-reactive protein (CRP) and anti-inflammatory cytokines (IL-4 and IL-10) were measured by ELISA. Cardiac mitochondrial activity ( cytochrome-C-oxidase), JNK activation and apoptosis (caspase-3, caspase-8 and caspase-9) were assessed. KEY FINDINGS: SIGNIFICANCE:
Arjunolic acid attenuated sodium nitrite-induced cardiac damage in rats and restored the normal balance between pro- and anti-inflammatory cytokines. Moreover, arjunolic acid protected cardiac tissues from both extrinsic and intrinsic cell death pathways.
|
Authors | Mohammed M H Al-Gayyar, Abdullah Al Youssef, Iman O Sherif, Mohamed E E Shams, Ahmed Abbas |
Journal | Life sciences
(Life Sci)
Vol. 111
Issue 1-2
Pg. 18-26
(Aug 28 2014)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 25064822
(Publication Type: Journal Article)
|
Copyright | Copyright © 2014 Elsevier Inc. All rights reserved. |
Chemical References |
- Cytokines
- Interleukin-1beta
- Triterpenes
- Tumor Necrosis Factor-alpha
- Interleukin-10
- Interleukin-4
- arjunolic acid
- C-Reactive Protein
- Electron Transport Complex IV
- Sodium Nitrite
|
Topics |
- Administration, Oral
- Animals
- Apoptosis
(drug effects)
- C-Reactive Protein
(analysis)
- Cytokines
(analysis)
- Electron Transport Complex IV
(drug effects, metabolism)
- Heart
(drug effects)
- Interleukin-10
(analysis)
- Interleukin-1beta
(analysis)
- Interleukin-4
(analysis)
- MAP Kinase Signaling System
(drug effects)
- Male
- Mitochondria, Heart
(drug effects, metabolism)
- Myocardium
(chemistry)
- Rats
- Rats, Sprague-Dawley
- Sodium Nitrite
(administration & dosage, antagonists & inhibitors, toxicity)
- Triterpenes
(pharmacology)
- Tumor Necrosis Factor-alpha
(analysis)
|