Abstract |
Diosmetin (3', 5, 7-trihydroxy-4'-methoxyflavone), the aglycone part of the flavonoid glycosides diosmin occurs naturally in citrus fruit, was considered to exhibit anti-inflammatory and antioxidant properties. Our study aimed to investigate the effect of diosmetin in a murine model of cerulein-induced acute pancreatitis (AP). Experimental AP was induced in mice by seven intraperitoneal injection of cerulein (50 ug/kg) at hourly intervals. Diosmetin (100 mg/kg) or vehicle was pretreated 2 h before the first cerulein injection. After 6 h, 9 h, 12 h of the first cerulein injection, the severity of acute pancreatitis was evaluated biochemically and morphologically. Pretreatment with diosmetin significantly reduced serum levels of amylase and lipase; the histological injury; the secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6; myeloperoxidase (MPO) activity, trypsinogen activation peptide (TAP) level, the expression of inducible nitric oxide synthase (iNOS); and the nuclear factor (NF)-κB activation in cerulein-induced AP. This study showed that administration of diosmetin demonstrated a beneficial effect on the course of cerulein-induced AP in mice. Therefore, diosmetin may become a new therapeutic agent in future clinical trials for treatment of AP.
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Authors | Ge Yu, Rong Wan, Guojian Yin, Jie Xiong, Yanling Hu, Miao Xing, Xiaofeng Cang, Yuting Fan, Wenqin Xiao, Lei Qiu, Xingpeng Wang, Guoyong Hu |
Journal | International journal of clinical and experimental pathology
(Int J Clin Exp Pathol)
Vol. 7
Issue 5
Pg. 2133-42
( 2014)
ISSN: 1936-2625 [Electronic] United States |
PMID | 24966921
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents
- Antioxidants
- Flavonoids
- Inflammation Mediators
- NF-kappa B
- Ceruletide
- diosmetin
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Topics |
- Active Transport, Cell Nucleus
(drug effects)
- Acute Disease
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Antioxidants
(pharmacology)
- Ceruletide
- Cytoprotection
- Disease Models, Animal
- Flavonoids
(pharmacology)
- Inflammation Mediators
(antagonists & inhibitors, metabolism)
- Male
- Mice, Inbred C57BL
- NF-kappa B
(antagonists & inhibitors, metabolism)
- Oxidative Stress
(drug effects)
- Pancreas
(drug effects, metabolism, pathology)
- Pancreatitis
(chemically induced, metabolism, pathology, prevention & control)
- Severity of Illness Index
- Signal Transduction
(drug effects)
- Time Factors
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