Increased argonaute 2 expression in gliomas and its association with tumor progression and poor prognosis.

Abstract

BACKGROUND:

Previous studies have showed that argonaute 2 is a potential factor related to genesis of several cancers, however, there have been no reports concerning gliomas.

METHODS:

Paraffin specimens of 129 brain glioma cases were collected from a hospital affiliated to Binzhou Medical University from January 2008 to July 2013. We examined both argonaute 2 mRNA and protein expression by real-time quantitative PCR (qRT-PCR), Western blot analysis, and immunohistochemistry (IHC). The survival curves of the patients were determined using the Kaplan-Meier method and Cox regression, and the log-rank test was used for statistical evaluations.

RESULTS:

Both argonaute 2 mRNA and protein were upregulated in high-grade when compared to low-grade tumor tissues. Multivariate analysis revealed that argonaute 2 protein expression was independently associated with the overall survival (HR=4.587, 95% CI: 3.001-6.993; P=0.002), and that argonaute 2 protein expression and WHO grading were independent prognostic factors for progression-free survival (HR=4.792, 95% CI: 3.993-5.672; P<0.001, and HR=2.109, 95% CI: 1.278-8.229; P=0.039, respectively). Kaplan-Meier analysis with the log-rank test indicated that high argonaute 2 protein expression had a significant impact on overall survival (P=0.0169) and progression-free survival (P=0.0324).

CONCLUSIONS:

The present study showed that argonaute 2 expression is up-regulated in gliomas. Argonaute 2 might also serve as a novel prognostic marker.

• Authors: Bo Feng, Peng Hu, Shu-Jun Lu, Jin-Bo Chen, Ru-Li Ge
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 15 Issue 9 Pg. 4079-83 ( 2014) ISSN: 2476-762X [Electronic] Thailand
• PMID: 24935600 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AGO2 protein, human
• Argonaute Proteins
• Biomarkers, Tumor
• RNA, Messenger

Topics

• Adult
• Aged
• Argonaute Proteins (biosynthesis)
• Biomarkers, Tumor (genetics)
• Brain Neoplasms (genetics, mortality, pathology)
• Disease Progression
• Disease-Free Survival
• Female
• Glioma (genetics, mortality, pathology)
• Humans
• Immunohistochemistry
• Kaplan-Meier Estimate
• Male
• Middle Aged
• Proportional Hazards Models
• RNA, Messenger (genetics)
• Tissue Preservation