Activation of SIRT1 by curcumin blocks the neurotoxicity of amyloid-β25-35 in rat cortical neurons.

Abstract

As one of the most important hallmarks of Alzheimer's disease (AD), β-amyloid (Aβ) plays important roles in inducing reactive oxygen species (ROS) generation, mitochondrial dysfunction and apoptotic cell death in neurons. Curcumin extracted from the yellow pigments spice plant turmeric shows multiplied bioactivities such as antioxidant and anti-apoptosis properties in vitro and in vivo. In the present study, the neuroprotective effect of curcumin against Aβ25-35-induced cell death in cultured cortical neurons was investigated. We found that pretreatment of curcumin prevented the cultured cortical neurons from Aβ25-35-induced cell toxicity. In addition, curcumin improved mitochondrial membrane potential (ΔΨm), decreased ROS generation and inhibited apoptotic cell death in Aβ25-35 treated neurons. Furthermore, we found that application of curcumin activated the expression of SIRT1 and subsequently decreased the expression of Bax in the presence of Aβ25-35. The protective effect of curcumin was blocked by SIRT1 siRNA. Taken together, our results suggest that activation of SIRT1 is involved in the neuroprotective action of curcumin.

• Authors: Qinru Sun, Ning Jia, Weixi Wang, Hui Jin, Jiehua Xu, Haitao Hu
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 448 Issue 1 Pg. 89-94 (May 23 2014) ISSN: 1090-2104 [Electronic] United States
• PMID: 24755072 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Amyloid beta-Peptides
• Neuroprotective Agents
• Peptide Fragments
• Reactive Oxygen Species
• amyloid beta-protein (25-35)
• bcl-2-Associated X Protein
• Sirt1 protein, rat
• Sirtuin 1
• Curcumin

Topics

• Amyloid beta-Peptides (toxicity)
• Animals
• Apoptosis (drug effects)
• Cell Death (drug effects)
• Cells, Cultured
• Curcumin (pharmacology)
• Membrane Potential, Mitochondrial (drug effects)
• Neurons (drug effects)
• Neuroprotective Agents (pharmacology)
• Neurotoxicity Syndromes
• Peptide Fragments (toxicity)
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species (metabolism)
• Sirtuin 1 (biosynthesis, metabolism)
• bcl-2-Associated X Protein (biosynthesis)