Immunization with recombinant 3-1E protein in AbISCO®-300 adjuvant induced protective immunity against Eimeria acervulina infection in chickens.

Abstract

Immunostimulating complexes (ISCOMs), a kind of novel antigen presenting system, could enhance immune protection by antigen presentation. AbISCO®-300 comprising purified saponin, cholesterol and phosphatidyl choline is an effective ISCOM adjuvant. To evaluate the immune protection of recombinant 3-1E protein against Eimeria acervulina infection, chickens were immunized with recombinant 3-1E protein in combination with AbISCO®-300 or recombinant 3-1E protein alone in this study. The protective immunity was assessed with body weight gain, fecal oocyst output, detection of intestinal IgA positive cells and percentages of CD3(+), CD4(+) or CD8(+) intestinal intraepithelial lymphocytes (IELs). Chickens vaccinated with different doses of recombinant 3-1E protein plus AbISCO®-300 showed higher percentages of CD3(+), CD4(+), and CD8(+) intestinal IELs, increased positive expression rate of intestinal IgA, increased body weight gains and decreased oocyst shedding compared with recombinant 3-1E protein-only vaccinated groups. The results showed that immunization with various doses of the recombinant 3-1E protein in AbISCO®-300 adjuvant enhanced immune protection against avian coccidiosis.

• Authors: Ying Zhang, Lixia Wang, Wenke Ruan, Jianjun Zhang, Peng Yao, Sai Zhou, Jian An
• Journal: Experimental parasitology (Exp Parasitol) Vol. 141 Pg. 75-81 (Jun 2014) ISSN: 1090-2449 [Electronic] United States
• PMID: 24675417 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Immunoglobulin A, Secretory
• Ki-1 Antigen
• Protozoan Proteins
• Protozoan Vaccines
• Recombinant Proteins

Topics

• Adjuvants, Immunologic
• Animals
• CD4 Lymphocyte Count (veterinary)
• CD8-Positive T-Lymphocytes
• Chickens (parasitology)
• Coccidiosis (parasitology, prevention & control, veterinary)
• Eimeria (immunology)
• Feces (parasitology)
• Flow Cytometry (veterinary)
• Immunoglobulin A, Secretory (analysis)
• Immunohistochemistry (veterinary)
• Intestinal Mucosa (cytology, immunology)
• Ki-1 Antigen (blood)
• Lymphocyte Count (veterinary)
• Male
• Parasite Egg Count (veterinary)
• Poultry Diseases (parasitology, prevention & control)
• Protozoan Proteins (genetics, immunology)
• Protozoan Vaccines
• Random Allocation
• Recombinant Proteins (genetics, immunology)
• Weight Gain