Cysteinyl leukotriene receptor antagonist regulates allergic airway inflammation in an organ- and cytokine-specific manner.

Abstract

BACKGROUND:

Cysteinyl leukotrienes (cys-LTs) are very important factors in the pathophysiology of bronchial asthma. Cys-LT receptor antagonists (LTRAs) decrease allergic airway inflammation. The aim of the present study was to determine the differential effects of LTRAs and corticosteroids on allergic airway inflammation and allergen-specific cytokine production from lymphoid tissues using a murine model of asthma.

MATERIAL AND METHODS:

Four groups of female BALB/c mice [control (Cont); Dermatophagoides farinae allergen-sensitized (AS); pranlukast (Prl), an LTRA-treated AS; and dexamethasone (Dex)-treated AS] were examined. Lung pathology and cytokine production by prepared mononuclear cells isolated from mediastinal lymph nodes (MLNs) and spleen were compared among these groups.

RESULTS:

AS mice exhibited allergic airway inflammation and significant increases in allergen-specific Th1 and Th2 cytokines in MLNs and spleen. Prl-treated mice showed significant attenuation of allergic airway inflammation concomitant with reduction of Th2 cytokines and IFN-g in MLNs but not in spleen. In contrast, Dex significantly decreased Th1 and Th2 cytokines in MLNs and also decreased them (except IL-13 and IL-2) in spleen.

CONCLUSIONS:

The inflammatory effects of cys-LTs could differ in lymphoid organs. LTRAs potentially regulate allergic airway inflammation in an organ- and cytokine-specific manner, while systemic corticosteroid shows nonspecific effects.

• Authors: Tetsuya Kawano, Hiroto Matsuse, Tomoko Tsuchida, Susumu Fukahori, Chizu Fukushima, Tomoya Nishino, Shigeru Kohno
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 20 Pg. 297-302 (Feb 22 2014) ISSN: 1643-3750 [Electronic] United States
• PMID: 24561545 (Publication Type: Journal Article)

Chemical References

• Adrenal Cortex Hormones
• Chromones
• Cytokines
• Leukotriene Antagonists
• Receptors, Leukotriene
• leukotriene D4 receptor
• pranlukast

Topics

• Adrenal Cortex Hormones (pharmacology)
• Analysis of Variance
• Animals
• Asthma (metabolism, physiopathology)
• Bronchoalveolar Lavage Fluid (cytology)
• Cell Count
• Chromones (pharmacology)
• Cytokines (metabolism)
• Female
• Leukocytes, Mononuclear (drug effects, metabolism)
• Leukotriene Antagonists (pharmacology)
• Lung (pathology)
• Lymph Nodes (cytology)
• Mice
• Mice, Inbred BALB C
• Receptors, Leukotriene (metabolism)
• Spleen (cytology)