Prenatal exposure to lipopolysaccharide combined with pre- and postnatal high-fat diet result in lowered blood pressure and insulin resistance in offspring rats.

Abstract

BACKGROUND:

Adult metabolic syndrome may in part have origins in fetal or early life. This study was designed to explore the effect of prenatal exposure to lipopolysaccharide and high-fat diet on metabolic syndrome in offspring rats.

METHODS:

32 pregnant rats were randomly divided into four groups, including Control group; LPS group (pregnant rats were injected with LPS 0.4 mg/kg intraperitoneally on the 8(th), 10(th) and 12(th) day of pregnancy); High-fat group (maternal rats had high-fat diet during pregnancy and lactation period, and their pups also had high-fat diet up to the third month of life); LPS + High-fat group (rats were exposed to the identical experimental scheme with LPS group and High-fat group).

RESULTS:

Blood pressure elevated in LPS group and High-fat group, reduced in LPS+High-fat group, accompanied by the increase of serum leptin level in LPS and High-fat group and increase of serum IL-6, TNF-a in High-fat group; both serum insulin and cholesterol increased in High-fat and LPS+High-fat group, as well as insulin in LPS group. HOMA-IR value increased in LPS, High-fat and LPS+High-fat group, and QUICKI decreased in these groups; H-E staining showed morphologically pathological changes in thoracic aorta and liver tissue in the three groups. Increased serum alanine and aspartate aminotransferase suggest impaired liver function in LPS+High-fat group.

CONCLUSION/SIGNIFICANCE:

Prenatal exposure to lipopolysaccharide combined with pre- and postnatal high-fat diet result in lowered blood pressure, insulin resistance and impaired liver function in three-month old offspring rats. The lowered blood pressure might benefit from the predictive adaptive response to prenatal inflammation.

• Authors: Xue-Qin Hao, Jing-Xia Du, Yan Li, Meng Li, Shou-Yan Zhang
• Journal: PloS one (PLoS One) Vol. 9 Issue 2 Pg. e88127 ( 2014) ISSN: 1932-6203 [Electronic] United States
• PMID: 24498431 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• Cytokines
• Insulin
• Lipopolysaccharides
• Triglycerides
• Cholesterol

Topics

• Animals
• Animals, Newborn
• Blood Glucose (metabolism)
• Blood Pressure (drug effects)
• Body Weight
• Cholesterol (metabolism)
• Cytokines (metabolism)
• Diet, High-Fat (adverse effects)
• Enzyme-Linked Immunosorbent Assay
• Female
• Insulin (metabolism)
• Insulin Resistance
• Kidney Function Tests
• Lipopolysaccharides (adverse effects)
• Liver Function Tests
• Male
• Metabolic Syndrome (etiology, metabolism, pathology)
• Pregnancy
• Prenatal Exposure Delayed Effects (etiology)
• Rats
• Rats, Sprague-Dawley
• Triglycerides (metabolism)