Abstract | OBJECTIVE: APPROACH AND RESULTS: A wire-induced carotid injury model was performed in wild-type mice and mice with inducible SMC-specific deletion of the MR. Aldosterone did not affect re-endothelialization after injury in wild-type mice. Deletion of SMC-MR prevented the 79% increase in SMC proliferation induced by aldosterone after injury in MR-Intact littermates. Moreover, both injury-induced and aldosterone-enhanced vascular fibrosis were attenuated in SMC-specific MR knockout mice. Further exploration of the mechanism revealed that aldosterone-induced vascular remodeling is prevented by in vivo blockade of the placental growth factor-specific receptor, type 1 vascular endothelial growth factor receptor (VEGFR1), the receptor for placental growth factor. Immunohistochemistry of carotid vessels shows that the induction of VEGFR1 expression in SMC after vascular injury is attenuated by 72% in SMC-specific MR knockout mice. Moreover, aldosterone induction of vascular placental growth factor mRNA expression and protein release are also prevented in vessels lacking SMC-MR. CONCLUSIONS:
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Authors | Dafina Pruthi, Amy McCurley, Mark Aronovitz, Carol Galayda, S Ananth Karumanchi, Iris Z Jaffe |
Journal | Arteriosclerosis, thrombosis, and vascular biology
(Arterioscler Thromb Vasc Biol)
Vol. 34
Issue 2
Pg. 355-64
(Feb 2014)
ISSN: 1524-4636 [Electronic] United States |
PMID | 24311380
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies
- Pgf protein, mouse
- Pregnancy Proteins
- RNA, Messenger
- Receptors, Mineralocorticoid
- Placenta Growth Factor
- Aldosterone
- Vascular Endothelial Growth Factor Receptor-1
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Topics |
- Aldosterone
(pharmacology)
- Animals
- Antibodies
(pharmacology)
- Carotid Arteries
(drug effects, metabolism, pathology)
- Carotid Artery Injuries
(genetics, metabolism, pathology)
- Cell Proliferation
(drug effects)
- Disease Models, Animal
- Endothelial Cells
(drug effects, metabolism, pathology)
- Fibrosis
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Muscle, Smooth, Vascular
(drug effects, metabolism, pathology)
- Myocytes, Smooth Muscle
(drug effects, metabolism, pathology)
- Placenta Growth Factor
- Pregnancy Proteins
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Receptors, Mineralocorticoid
(agonists, deficiency, genetics)
- Time Factors
- Vascular Endothelial Growth Factor Receptor-1
(antagonists & inhibitors, metabolism)
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