Abstract | BACKGROUND: This study was performed to investigate whether ginseng has a protective effect in an experimental mouse model of cyclosporine-induced pancreatic injury. METHODS: Mice were treated with cyclosporine (30 mg/kg/day, subcutaneously) and Korean red ginseng extract (0.2 or 0.4 g/kg/day, oral gavage) for 4 weeks while on a 0.01% salt diet. The effect of ginseng on cyclosporine-induced pancreatic islet dysfunction was investigated by an intraperitoneal glucose tolerance test and measurements of serum insulin level, β cell area, macrophage infiltration, and apoptosis. Using an in vitro model, we further examined the effect of ginseng on a cyclosporine-treated insulin-secreting cell line. Oxidative stress was measured by the concentration of 8-hydroxy-2'-deoxyguanosine in serum, tissue sections, and culture media. RESULTS: CONCLUSIONS: The results of our in vivo and in vitro studies demonstrate that ginseng has a protective effect against cyclosporine-induced pancreatic β cell injury via reducing oxidative stress.
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Authors | Sun Woo Lim, Kyoung Chan Doh, Long Jin, Shang Guo Piao, Seong Beom Heo, Yu Fen Zheng, Soo Kyung Bae, Byung Ha Chung, Chul Woo Yang |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 8
Pg. e72685
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24009697
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Nitrites
- Plant Extracts
- Cyclosporine
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Topics |
- Administration, Oral
- Animals
- Apoptosis
(drug effects)
- Cyclosporine
(adverse effects)
- Disease Models, Animal
- Insulin-Secreting Cells
(drug effects)
- Islets of Langerhans
(drug effects, pathology)
- Kidney Function Tests
- Macrophages
(drug effects, pathology)
- Mice
- Nitrites
(metabolism)
- Oxidative Stress
(drug effects)
- Panax
(chemistry)
- Pancreas
(drug effects, pathology, physiopathology)
- Pancreatic Diseases
(chemically induced, drug therapy, metabolism)
- Plant Extracts
(administration & dosage)
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