Elevated fetal adipsin/acylation-stimulating protein (ASP) in obese pregnancy: novel placental secretion via Hofbauer cells.

Abstract

CONTEXT AND OBJECTIVE:

Obesity in pregnancy is associated with increased risks of obesity in the offspring. We investigated the relationship between obesity in pregnancy and circulating maternal and fetal levels of adipose tissue-derived factors adipsin and acylation stimulating protein (ASP) in lean and obese mothers.

DESIGN:

Paired peripheral and cord blood samples were taken. Paired fat and placenta tissue were taken for explant culture. Media were assayed for secreted adipsin and ASP. Clinical parameters assayed included fasting insulin, glucose, and adipsin.

SETTING:

The study was conducted at a university hospital maternity unit.

PATIENTS:

Patients included 35 lean [body mass index (BMI) 19-25 kg/m(2), mean age 32 years and 39 obese (BMI) > 30 kg/m(2), mean age 32.49 years] pregnant Caucasian women, delivered by cesarean section at term.

MAIN OUTCOME MEASURE:

Identification of placental macrophages [Hofbauer cells (HBCs)], as a source of adipsin and ASP was determined.

RESULTS:

HBCs secreted both adipsin and ASP. Cord levels of adipsin (1663.78 ± 52.76 pg/mL) and ASP (354.48 ± 17.17 ng/mL) were significantly elevated in the offspring of obese mothers compared with their lean controls [1354.66 ± 33.87 pg/mL and 302.63 ± 14.98 ng/mL, respectively (P < .05 for both)]. Placentae from obese mothers released significantly more adipsin and ASP than placentae from lean mothers [546.0 ± 44 pg/mL · g vs 284.56 ± 43 pg/mL · g and 5485.75 ± 163.32 ng/mL · g vs 2399.16 ± 181.83 ng/mL · g, respectively (P < .05 for both)]. Circulating fetal adipsin and ASP positively correlated with maternal BMI (r = 0.611, P < .0001, and r = 0.391, P < .05, respectively). Fetal adipsin correlated positively with maternal (r = 0.482, P < .01) and fetal homeostasis model assessment of insulin resistance (r = 0.465, P < .01).

CONCLUSIONS:

We demonstrate novel secretion of adipsin and ASP by placental HBCs.

• Authors: K Sivakumar, M F Bari, A Adaikalakoteswari, S Guller, M O Weickert, H S Randeva, D K Grammatopoulos, C C Bastie, M Vatish
• Journal: The Journal of clinical endocrinology and metabolism (J Clin Endocrinol Metab) Vol. 98 Issue 10 Pg. 4113-22 (Oct 2013) ISSN: 1945-7197 [Electronic] United States
• PMID: 23956345 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• C3 protein, human
• Complement C3
• Intercellular Signaling Peptides and Proteins
• Complement Factor D

Topics

• Adipose Tissue (metabolism)
• Adult
• Body Composition
• Body Mass Index
• Complement C3
• Complement Factor D (metabolism)
• Female
• Fetal Blood (metabolism)
• Humans
• Intercellular Signaling Peptides and Proteins (blood, metabolism)
• Macrophages (metabolism)
• Obesity (blood, metabolism)
• Placenta (metabolism)
• Pregnancy