Abstract | PURPOSE: The purpose of present studies was to determine the involvement of NFκB and STAT6 transcription factors in the production of cytokines by the fibroblasts and epithelial cells in conjunctiva. METHODS: RESULTS: Stimulated rat mast cells released TNF-α and IL-4. TNF-α induced NFκB activation in rat and human conjunctival fibroblasts and epithelial cells, and caused production and release of cytokines IL-8 and RANTES. IL-4 activation of STAT6 did not cause release of these cytokines. Only fibroblasts produced the eosinophil-recruiting cytokine, eotaxin-1, after treatment with TNF-α- plus IL-4. As observed in the cultured cells, allergic stimulation in the in vivo model caused degradation of IκB-α in conjunctiva, and infiltration of eosinophils and other inflammatory cells. CONCLUSION: Activated NFκB was found to be a major transcription factor for the release of cytokines from conjunctival cells and intensification of the allergic response. Inhibition of the NFκB pathway by therapeutic drugs may be an important objective for the treatment of human allergic conjunctivitis.
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Authors | Osamu Sakai, Yoshiyuki Tamada, Thomas R Shearer, Mitsuyoshi Azuma |
Journal | Current eye research
(Curr Eye Res)
Vol. 38
Issue 8
Pg. 825-34
(Aug 2013)
ISSN: 1460-2202 [Electronic] England |
PMID | 23621293
(Publication Type: Journal Article)
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Chemical References |
- Chemokine CCL11
- Chemokines
- Culture Media, Conditioned
- Interleukin-8
- NF-kappa B
- STAT6 Transcription Factor
- Tumor Necrosis Factor-alpha
- Interleukin-4
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Topics |
- Animals
- Cells, Cultured
- Chemokine CCL11
(immunology, metabolism)
- Chemokines
(immunology, metabolism)
- Conjunctiva
(cytology, immunology, metabolism)
- Conjunctivitis, Allergic
(immunology, metabolism)
- Culture Media, Conditioned
(pharmacology)
- Epithelial Cells
(cytology, immunology, metabolism)
- Fibroblasts
(cytology, immunology, metabolism)
- Interleukin-4
(immunology, metabolism)
- Interleukin-8
(immunology, metabolism)
- Male
- Mast Cells
(cytology, immunology, metabolism)
- NF-kappa B
(immunology, metabolism)
- Rats
- Rats, Sprague-Dawley
- STAT6 Transcription Factor
(metabolism)
- Tumor Necrosis Factor-alpha
(immunology, metabolism)
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