Abstract | OBJECTIVE: METHODS: Primary cultures of hippocampal neurons were used to select patients' sera that showed robust reactivity with the cell surface of neurons. The identity of the antigens was established by immunoprecipitation and mass spectrometry, and subsequently confirmed with cell-based assays, immunohistochemistry with teased rat sciatic nerve, and immunoabsorption experiments. RESULTS: Four of 46 sera from patients with CIDP reacted strongly against hippocampal neurons (8.6%) and paranodal structures on peripheral nerve. Two patients' sera precipitated contactin-1 (CNTN1), and 1 precipitated both CNTN1 and contactin-associated protein 1 (CASPR1). Reactivity against CNTN1 was confirmed in 2 cases, whereas the third reacted only when CNTN1 and CASPR1 were cotransfected. No other CIDP patient or any of the 104 controls with other neurological diseases tested positive. All 3 patients shared common clinical features, including advanced age, predominantly motor involvement, aggressive symptom onset, early axonal involvement, and poor response to intravenous immunoglobulin. INTERPRETATION:
Antibodies against the CNTN1/CASPR1 complex occur in a subset of patients with CIDP who share common clinical features. The finding of this biomarker may help to explain the symptoms of these patients and the heterogeneous response to therapy in CIDP.
|
Authors | Luis Querol, Gisela Nogales-Gadea, Ricard Rojas-Garcia, Eugenia Martinez-Hernandez, Jordi Diaz-Manera, Xavier Suárez-Calvet, Miquel Navas, Josefa Araque, Eduard Gallardo, Isabel Illa |
Journal | Annals of neurology
(Ann Neurol)
Vol. 73
Issue 3
Pg. 370-80
(Mar 2013)
ISSN: 1531-8249 [Electronic] United States |
PMID | 23280477
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2012 American Neurological Association. |
Chemical References |
- Antibodies
- CNTN1 protein, human
- CNTNAP2 protein, human
- Cell Adhesion Molecules, Neuronal
- Contactin 1
- Membrane Proteins
- Nerve Tissue Proteins
|
Topics |
- Aged
- Animals
- Animals, Newborn
- Antibodies
(blood, pharmacology)
- Cell Adhesion Molecules, Neuronal
(metabolism)
- Cells, Cultured
- Contactin 1
(immunology)
- Female
- Follow-Up Studies
- Hippocampus
(cytology, pathology)
- Humans
- Male
- Membrane Proteins
(metabolism)
- Middle Aged
- Nerve Tissue Proteins
(metabolism)
- Neural Conduction
(drug effects)
- Neurons
(drug effects, metabolism, pathology)
- Polyradiculoneuropathy, Chronic Inflammatory Demyelinating
(blood, immunology)
- Rats
- Rats, Inbred Lew
- Sciatic Nerve
(metabolism)
- Serum
- Statistics, Nonparametric
- Transfection
|