A natural antioxidant pine bark extract, Oligopin®, regulates the stress chaperone HSPB1 in human skeletal muscle cells: a proteomics approach.

Abstract	The gradual loss of muscle mass affecting all the elderly (sarcopenia) is most likely due to a decreased number and/or function of satellite cells. Accumulation of reactive oxygen species (ROS) has been clearly correlated to sarcopenia and could contribute to the impairment of satellite cell function. In this study, we analyzed the protective mechanism of action of a natural pine bark extract (Oligopin®) in human muscle satellite cells exposed to oxidative stress (H2O2). This polyphenol belongs to the flavonoid family and was able to abolish the H2 O2-induced apoptotic cell death. A large-scale proteomic strategy allowed us to identify several proteins that may function as early regulators of ROS-mediated events in muscle cells. Interestingly, we identified the stress chaperone heat shock protein beta-1, a main protector of muscle necrosis, as a target of Oligopin® and showed that this polyphenol was able to modulate its stress induced phosphorylation.
Authors	Sylvie Poussard, Amélie Pires-Alves, Ramata Diallo, Jean-William Dupuy, Elise Dargelos
Journal	Phytotherapy research : PTR (Phytother Res) Vol. 27 Issue 10 Pg. 1529-35 (Oct 2013) ISSN: 1099-1573 [Electronic] England
PMID	23192879 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright	Copyright © 2012 John Wiley & Sons, Ltd.
Chemical References	Antioxidants HSP27 Heat-Shock Proteins HSPB1 protein, human Heat-Shock Proteins Molecular Chaperones Oligopin Plant Extracts Polyphenols Hydrogen Peroxide
Topics	Antioxidants (pharmacology) Apoptosis (drug effects) Cell Line HSP27 Heat-Shock Proteins (metabolism) Heat-Shock Proteins Humans Hydrogen Peroxide (pharmacology) Molecular Chaperones Oxidative Stress (drug effects) Phosphorylation Pinus (chemistry) Plant Bark (chemistry) Plant Extracts (pharmacology) Polyphenols (pharmacology) Proteomics Sarcopenia Satellite Cells, Skeletal Muscle (drug effects, metabolism)

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