Abstract | PURPOSE: EXPERIMENTAL DESIGN: Serum glutamate levels were measured in normal men (n = 60) and patients with primary prostate cancer (n = 197) or mCRPCa (n = 109). GRM1 expression in prostatic tissues was examined using immunohistochemistry (IHC). Cell growth, migration, and invasion were determined using cell cytotoxicity and modified Boyden chamber assays, respectively. Apoptosis was detected using immunoblotting against cleaved caspases, PARP, and γ-H2AX. RESULTS: Univariate and multivariate analyses showed significantly higher serum glutamate levels in Gleason score ≥ 8 than in the Gleason score ≤ 7 and in African Americans than in the Caucasian Americans. African Americans with mCRPCa had significantly higher serum glutamate levels than those with primary prostate cancer or benign prostate. However, in Caucasian Americans, serum glutamate levels were similar in normal research subjects and patients with mCRPC. IHC showed weak or no expression of GRM1 in luminal acinar epithelial cells of normal or hyperplastic glands but high expression in primary or metastatic prostate cancer tissues. Glutamate deprivation or blockade decreased prostate cancer cells' proliferation, migration, and invasion and led to apoptotic cell death. CONCLUSIONS:
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Authors | Shahriar Koochekpour, Sunipa Majumdar, Gissou Azabdaftari, Kristopher Attwood, Ray Scioneaux, Dhatchayini Subramani, Charles Manhardt, Giovanni D Lorusso, Stacey S Willard, Hillary Thompson, Mojgan Shourideh, Katayoon Rezaei, Oliver Sartor, James L Mohler, Robert L Vessella |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 18
Issue 21
Pg. 5888-901
(Nov 01 2012)
ISSN: 1557-3265 [Electronic] United States |
PMID | 23072969
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, P.H.S.)
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Copyright | ©2012 AACR |
Chemical References |
- Receptors, Metabotropic Glutamate
- Glutamic Acid
- Riluzole
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Topics |
- Adult
- Black or African American
- Apoptosis
(drug effects)
- Case-Control Studies
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Glutamic Acid
(blood)
- Humans
- Male
- Middle Aged
- Neoplasm Grading
- Prostate
(metabolism)
- Prostatic Neoplasms
(blood, metabolism, pathology)
- Receptors, Metabotropic Glutamate
(antagonists & inhibitors, metabolism)
- Riluzole
(pharmacology)
- White People
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