Abstract |
Cardiac hypertrophy is initiated as an adaptive response to sustained overload but progresses pathologically as heart failure ensues. Here we report that genetic loss of APJ, a G-protein-coupled receptor, confers resistance to chronic pressure overload by markedly reducing myocardial hypertrophy and heart failure. In contrast, mice lacking apelin (the endogenous APJ ligand) remain sensitive, suggesting an apelin-independent function of APJ. Freshly isolated APJ-null cardiomyocytes exhibit an attenuated response to stretch, indicating that APJ is a mechanosensor. Activation of APJ by stretch increases cardiomyocyte cell size and induces molecular markers of hypertrophy. Whereas apelin stimulates APJ to activate Gαi and elicits a protective response, stretch signals in an APJ-dependent, G-protein-independent fashion to induce hypertrophy. Stretch-mediated hypertrophy is prevented by knockdown of β- arrestins or by pharmacological doses of apelin acting through Gαi. Taken together, our data indicate that APJ is a bifunctional receptor for both mechanical stretch and the endogenous peptide apelin. By sensing the balance between these stimuli, APJ occupies a pivotal point linking sustained overload to cardiomyocyte hypertrophy.
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Authors | Maria Cecilia Scimia, Cecilia Hurtado, Saugata Ray, Scott Metzler, Ke Wei, Jianming Wang, Chris E Woods, Nicole H Purcell, Daniele Catalucci, Takeshi Akasaka, Orlando F Bueno, George P Vlasuk, Perla Kaliman, Rolf Bodmer, Layton H Smith, Euan Ashley, Mark Mercola, Joan Heller Brown, Pilar Ruiz-Lozano |
Journal | Nature
(Nature)
Vol. 488
Issue 7411
Pg. 394-8
(Aug 16 2012)
ISSN: 1476-4687 [Electronic] England |
PMID | 22810587
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adipokines
- Apelin
- Apelin Receptors
- Apln protein, mouse
- Aplnr protein, mouse
- Arrestins
- Intercellular Signaling Peptides and Proteins
- Receptors, G-Protein-Coupled
- beta-Arrestins
- GTP-Binding Protein alpha Subunits, Gi-Go
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Topics |
- Adipokines
- Animals
- Aorta
(pathology)
- Apelin
- Apelin Receptors
- Arrestins
(deficiency, genetics, metabolism)
- Blood Pressure
- Cardiomegaly
(metabolism, pathology, physiopathology, prevention & control)
- Female
- GTP-Binding Protein alpha Subunits, Gi-Go
(metabolism)
- Intercellular Signaling Peptides and Proteins
(deficiency, genetics, metabolism, pharmacology)
- Male
- Mechanoreceptors
(metabolism)
- Mechanotransduction, Cellular
(drug effects, physiology)
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Myocytes, Cardiac
(drug effects, pathology)
- Receptors, G-Protein-Coupled
(agonists, deficiency, genetics, metabolism)
- Signal Transduction
(drug effects)
- beta-Arrestins
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