Medical ozone therapy decreases postoperative uterine adhesion formation in rats.

Abstract

PURPOSE:

Various studies have been performed to find out novel treatment strategies to prevent postoperative adhesion formation. Ozone therapy (OT) is shown to reduce inflammation in several pathological conditions. Therefore, we aimed to evaluate the efficacy of OT in a rat model of experimental uterine adhesion (EUA).

METHODS:

Thirty female Wistar rats (200-250 g) were divided into three groups: sham, EUA and EUA+OT. EUA and EUA+OT groups were subjected to the postoperative adhesion procedure by bipolar coagulation on the uterine horns and corresponding pelvic sidewall parietal peritoneum. EUA+OT group received 0.7 mg/kg daily single dose for 3 days of ozone/oxygen mixture intraperitoneally after adhesion induction. All animals were killed on the 7th day and uterine adhesions were scored. Uterine tissues and peritoneal washing fluid were harvested for all analyses.

RESULTS:

Uterine malondialdehyde levels in the EUA group were significantly higher compared to the other groups. However, in the EUA group, uterine superoxide dismutase and glutathione peroxidase activities were lower than in other groups. Peritoneal fluid TNF-α levels were found to be significantly different for all groups (p < 0.001). Macroscopic total adhesion score was significantly higher in the EUA group compared to the other groups (p < 0.001). But, total score in the EUA+OT group was lower than in the EUA group (p = 0.006).

CONCLUSIONS:

Medical OT prevents postoperative uterine adhesions by modulating TNF-α levels and oxidative/antioxidative status in an experimental uterine adhesion model.

• Authors: Bulent Uysal, Suzi Demirbag, Yavuz Poyrazoglu, Tuncer Cayci, Narter Yesildaglar, Ahmet Guven, Ilhami Sürer, Ahmet Korkmaz
• Journal: Archives of gynecology and obstetrics (Arch Gynecol Obstet) Vol. 286 Issue 5 Pg. 1201-7 (Nov 2012) ISSN: 1432-0711 [Electronic] Germany
• PMID: 22740111 (Publication Type: Journal Article)

Chemical References

• Oxidants, Photochemical
• Tumor Necrosis Factor-alpha
• Malondialdehyde
• Ozone
• Glutathione Peroxidase
• Superoxide Dismutase

Topics

• Animals
• Ascitic Fluid (metabolism)
• Disease Models, Animal
• Electrocoagulation
• Female
• Glutathione Peroxidase (metabolism)
• Inflammation (metabolism, prevention & control)
• Malondialdehyde (metabolism)
• Oxidants, Photochemical (therapeutic use)
• Oxidative Stress
• Ozone (therapeutic use)
• Peritoneum (surgery)
• Postoperative Complications (metabolism, pathology, prevention & control)
• Rats
• Rats, Wistar
• Superoxide Dismutase (metabolism)
• Tissue Adhesions (metabolism, pathology, prevention & control)
• Tumor Necrosis Factor-alpha (metabolism)
• Uterus (metabolism, surgery)