Abstract | INTRODUCTION: Early detection of malignancies in the lung by less-invasive methods aims at achieving efficient intervention and subsequently a reduction of the high mortality rate. We investigated whether biomarker analysis in endobronchial epithelial-lining fluid (ELF) collected by bronchoscopic microsampling (BMS) may be useful for a definitive preoperative diagnosis. METHODS: ELF was collected from subsegmental bronchi close to the indeterminate pulmonary nodule, which was detected by computed tomography, and from the contralateral lung. Diagnosis was confirmed by transbronchial biopsy or surgery. The study includes 142 ELF samples from 51 non-small-cell lung cancer patients and 20 benign cases. Microarray analysis was done with a patient subset (n = 15) to narrow down genes associated with a malignant phenotype. Thirteen potential biomarkers have been further analyzed by quantitative real-time polymerases chain reaction in an independent patient cohort (n = 56). RESULTS: CONCLUSIONS: Our study suggests that the analysis of specific biomarkers in ELF collected by BMS could be a potentially useful adjunct to other diagnostic techniques aiming at the preoperative diagnosis of malignant pulmonary nodules.
|
Authors | Nicolas Kahn, Michael Meister, Ralf Eberhardt, Thomas Muley, Philipp A Schnabel, Christian Bender, Marc Johannes, Denise Keitel, Holger Sültmann, Felix J F Herth, Ruprecht Kuner |
Journal | Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
(J Thorac Oncol)
Vol. 7
Issue 6
Pg. 1001-8
(Jun 2012)
ISSN: 1556-1380 [Electronic] United States |
PMID | 22588153
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Biomarkers, Tumor
- RNA, Neoplasm
- Tenascin
|
Topics |
- Aged
- Biomarkers, Tumor
(analysis, genetics)
- Biopsy
- Bronchoalveolar Lavage Fluid
(chemistry, cytology)
- Bronchoscopy
- Carcinoma, Non-Small-Cell Lung
(diagnosis, genetics, metabolism)
- Early Diagnosis
- Female
- Follow-Up Studies
- Gene Expression Regulation, Neoplastic
- Humans
- Lung
(diagnostic imaging, pathology)
- Lung Neoplasms
(diagnosis, genetics, metabolism)
- Male
- Middle Aged
- Neoplasm Staging
- Phenotype
- Predictive Value of Tests
- RNA, Neoplasm
(analysis, genetics)
- Real-Time Polymerase Chain Reaction
- Respiratory Mucosa
(metabolism, pathology)
- Tenascin
(biosynthesis, genetics)
- Time Factors
- Tomography, X-Ray Computed
|