[Regulating action of iron regulatory protein-2 in iron metabolism of lung cancer].

Abstract

OBJECTIVE:

To discuss the regulating mechanism of iron regulatory protein-2 (IRP2) in the iron metabolism of lung cancer.

METHODS:

The cultured A549 cells were divided into 3 groups: liposome group (including liposomes 20 mg/L), random oligonucleotide group (SCODN group) and antisense oligonucleotide group (ASODN group). And the liposome-mediated transfection was employed with the liposome and SCODN groups as controls. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to examine the mRNA and protein expressions of iron metabolism-related transferring (Tf), transferrin receptor (TfR) and ferritin (Fn) genes, etc.

RESULTS:

After a 48-hour transfection, the mRNA expression of Tf had no statistically significant difference among three groups (F = 2.18, P = 0.078); the mRNA expression of TfR in the ASODN group was significantly lower than that in the liposome and SCODN groups (P < 0.05). The expression of Fn mRNA in the ASODN group (0.56 ± 0.06) was higher than that in the liposome (0.36 ± 0.05) and SCODN groups (0.39 ± 0.03) (P < 0.05). After a 48-hour transfection, the IRP2 protein expression of the ASODN group was significantly lower than those of the liposome and SCODN groups (P < 0.05). The Tf protein expression was not statistically different in three groups (F = 2.67, P = 0.088). The TfR protein expression of the ASODN group was lower than those of the liposome and SCODN groups (P < 0.05). And the Fn protein expression of the ASODN group was higher than those of the liposome and SCODN groups (P < 0.05).

CONCLUSION:

IRP2 may affect the expressions of TfR and Fn in lung adenocarcinoma A549 cells by changing the amount of protein and regulating the iron metabolism.

• Authors: Zhe Cheng, Yu-feng Liu, Yong-na Song, Ling-ling Dai, Yan Kang, Jie Xia, Ji-ming Si, Chun-yan Chen
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 91 Issue 28 Pg. 1992-5 (Jul 26 2011) ISSN: 0376-2491 [Print] China
• PMID: 22093897 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Liposomes
• Oligodeoxyribonucleotides, Antisense
• Receptors, Transferrin
• Ferritins
• Iron
• IREB2 protein, human
• Iron Regulatory Protein 2

Topics

• Adenocarcinoma (metabolism)
• Adenocarcinoma of Lung
• Cell Line, Tumor
• Ferritins (metabolism)
• Humans
• Iron (metabolism)
• Iron Regulatory Protein 2 (genetics, metabolism)
• Liposomes (administration & dosage)
• Lung Neoplasms (metabolism)
• Oligodeoxyribonucleotides, Antisense (genetics)
• Receptors, Transferrin (metabolism)