The effects of grape seeds polyphenols on SKH-1 mice skin irradiated with multiple doses of UV-B.

Abstract

The study investigated the protective activity of red grape seeds (Vitis vinifera L, Burgund Mare variety) (BM) extracts in vivo on multiple doses of ultraviolet radiation (UV)-B-induced deleterious effects in SKH-1 mice skin. Eighty 8-weeks-old female SKH-1 mice were divided into 8 groups: control, vehicle, UV-B irradiated, vehicle+UV-B irradiated, BM 2.5mg polyphenols (PF)/cm(2)+UV-B irradiated, BM 4 mg PF/cm(2)+UV-B irradiated, UV-B+BM 2.5mg PF/cm(2), UV-B+BM 4 mg PF/cm(2). The extract was applied topically before or after each UV-B exposure (240 mJ/cm(2)), for 10 days consecutively. The antioxidant activity of BM extract is higher than gallic acid (k(BM)=0.017, k(gallic acid)=0.013). Multiple doses of UV-B generated the formation of cyclobutane pyrimidine dimers (CPDs) and sunburn cells, increased glutathione peroxidase (GPx) and catalase (CAT) activities respectively glutathione (GSH) and IL-1β levels in skin. In group treated with 2.5mg PF/cm(2) before UV-B irradiation BM extract inhibited UV-B-induced sunburn cells, restored the superoxide dismutase (MnSOD) activity, increased insignificantly CAT and GPx activities and reduced IL-1β level. The BM 4.0 mg PF/cm(2) treatment decreased GSH level and reduced the percentage of CPDs positive cells in skin. Both doses of BM extract administered after UV-B irradiation increased the MnSOD and GPx activities and reduced the formation of sunburn cells in skin. Our results suggest that BM extract might be a potential chemo-preventive candidate in reducing the oxidative stress and apoptosis induced by multiple doses of UV-B in skin.

• Authors: Adriana Filip, Doina Daicoviciu, Simona Clichici, Pompei Bolfa, Cornel Catoi, Ioana Baldea, Laura Bolojan, Diana Olteanu, Adriana Muresan, I D Postescu
• Journal: Journal of photochemistry and photobiology. B, Biology (J Photochem Photobiol B) Vol. 105 Issue 2 Pg. 133-42 (Nov 03 2011) ISSN: 1873-2682 [Electronic] Switzerland
• PMID: 21925895 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Antioxidants
• Cytokines
• Plant Extracts
• Polyphenols
• Pyrimidine Dimers
• Malondialdehyde
• Glutathione

Topics

• Animals
• Antioxidants (pharmacology)
• Cytokines (metabolism)
• Dose-Response Relationship, Radiation
• Female
• Glutathione (metabolism)
• Malondialdehyde (metabolism)
• Mice
• Mice, Hairless
• Oxidative Stress (drug effects)
• Plant Extracts (pharmacology)
• Polyphenols (pharmacology)
• Pyrimidine Dimers (metabolism)
• Seeds (chemistry)
• Skin (drug effects, enzymology, metabolism, radiation effects)
• Sunburn (pathology, prevention & control)
• Ultraviolet Rays (adverse effects)
• Vitis (chemistry)