Proteins in leaked amniotic fluid as biomarkers diagnostic for prelabor rupture of membranes.

Abstract

PURPOSE:

Early diagnosis of prelabor rupture of membranes (PROM) is essential to protect mother and fetus from intra-uterus infection and preterm birth. A simple and rapid bedside test would help clinicians confirm the diagnosis for early treatment.

EXPERIMENTAL DESIGN:

A protein array was used to screen cervical-vaginal fluid (CVF) and amniotic fluid (AF) samples collected from normal and PROM pregnant women. Enzyme-linked immunosorbent assay was used to quantify two novel and potentially useful analytes, soluble intercellular adhesion molecule-1 (sICAM-1) and Axl receptor tyrosine kinase (Axl).

RESULTS:

The mean concentration of sICAM-1 and Axl was 85 and 482 times higher separately in 30 healthy AF samples than in 110 CVF samples of normal pregnancies. Comparing 110 CVF samples of PROM/Preterm PROM with 110 CVF samples of normal pregnancies, the diagnostic value for PROM was demonstrated by their high sensitivity and specificity (96.4 and 92.7%, respectively, for sICAM-1, and 92.4 and 90.4%, respectively, for Axl).

CONCLUSIONS AND CLINICAL RELEVANCE:

The results indicate that sICAM-1 and Axl in AF leaked to vagina are sensitive and specific biomarkers for the diagnosis of PROM. Furthermore, sICAM-1 or Axl can be developed into a rapid strip test for bedside use.

• Authors: Tao Wang, Rong Zhou, Lin Zhang, Yanyun Wang, Chang ping Song, Wei Lin, Xiaoyu Niu, Yong Lin, Huaizhong Hu
• Journal: Proteomics. Clinical applications (Proteomics Clin Appl) Vol. 5 Issue 7-8 Pg. 415-21 (Aug 2011) ISSN: 1862-8354 [Electronic] Germany
• PMID: 21656911 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Biomarkers
• Proto-Oncogene Proteins
• Intercellular Adhesion Molecule-1
• Receptor Protein-Tyrosine Kinases
• Axl Receptor Tyrosine Kinase
• AXL protein, human

Topics

• Amniotic Fluid (chemistry)
• Biomarkers (analysis)
• Enzyme-Linked Immunosorbent Assay
• Female
• Fetal Membranes, Premature Rupture (diagnosis, metabolism, pathology, prevention & control)
• Fetus
• Humans
• Infant, Newborn
• Infant, Premature
• Infant, Premature, Diseases (diagnosis, pathology)
• Intercellular Adhesion Molecule-1 (analysis)
• Pregnancy
• Premature Birth (diagnosis, prevention & control)
• Proto-Oncogene Proteins (analysis)
• Receptor Protein-Tyrosine Kinases (analysis)
• Sensitivity and Specificity
• Vagina (chemistry)
• Axl Receptor Tyrosine Kinase