Abstract | OBJECTIVES: DESIGN: Retrospective correlative analysis. SETTING: University teaching hospital, Hong Kong. PATIENTS: Patients diagnosed with histologically proven primary glioblastoma in the period of March 2005 to June 2007 were recruited. Genomic DNA was isolated from formalin-fixed and paraffin-embedded sections of glioblastoma tissues. Methylation-specific polymerase chain reaction for O(6)Methylguanine DNA methyltransferase was performed. Patients' information at presentation was collected (age, performance status, steroid use, extent of resection, complications, radiotherapy data, use of chemotherapy). Primary outcome was measured by overall survival while secondary outcome was measured by progression-free survival. Overall and progression-free survivals were estimated by the Kaplan-Meier technique. Outcomes were assessed for groups with and without concomitant chemoradiotherapy and for groups with and without O(6)Methylguanine DNA methyltransferase methylation. RESULTS: A total of 35 glioblastoma patients were recruited; 27 were male and 8 female. Their mean age was 50 years. In all, 17 received concomitant chemoradiotherapy, and 18 received radiotherapy only. Their median overall survival was 12 (range, 7-17) months and the median progression-free survival was 5 (range, 3-6) months. In the radiotherapy alone group, the median progression-free survival and overall survival was 4 (range, 3-5) months and 6 (range, 2-10) months, respectively. In the concomitant radiochemotherapy group, the median progression-free survival and overall survival was 6 (range, 2-10) months and 13 (range, 8-18) months, respectively. Fifteen (43%) of the tumour samples showed methylation of O(6)Methylguanine DNA methyltransferase. There was a trend towards overall longer survival in the group with methylated tumours compared to those with unmethylated tumours; respective values for median survival (ranges) were 17 (13-21) versus 10 (6-14) months (P=0.105). CONCLUSIONS:
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Authors | Danny T M Chan, Michael K M Kam, Brigette B Y Ma, Stephanie C P Ng, Jesse C S Pang, Claire K Y Lau, Deyond Y W Siu, Benedict S L Ng, X L Zhu, George G Chen, H K Ng, W S Poon |
Journal | Hong Kong medical journal = Xianggang yi xue za zhi
(Hong Kong Med J)
Vol. 17
Issue 3
Pg. 184-8
(Jun 2011)
ISSN: 1024-2708 [Print] China |
PMID | 21636865
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- O(6)-Methylguanine-DNA Methyltransferase
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Topics |
- Asian People
- Chemotherapy, Adjuvant
(methods)
- Combined Modality Therapy
- DNA Methylation
- Disease-Free Survival
- Female
- Glioblastoma
(drug therapy, genetics, radiotherapy)
- Hong Kong
- Hospitals, University
- Humans
- Kaplan-Meier Estimate
- Male
- Middle Aged
- O(6)-Methylguanine-DNA Methyltransferase
(genetics)
- Polymerase Chain Reaction
- Prognosis
- Retrospective Studies
- Survival Rate
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