Gene silencing activity of siRNA polyplexes based on biodegradable polymers.

Abstract

Cationic polymers are used as non-viral vectors for nucleic acid delivery. In this study, two biodegradable cationic polymers were evaluated for the purpose of siRNA delivery: pHPMA-MPPM (poly((2-hydroxypropyl) methacrylamide 1-methyl-2-piperidine methanol)) and TMC (O-methyl-free N,N,N-trimethylated chitosan). The silencing activity and the cellular cytotoxicity of polyplexes based on these biodegradable polymers were compared with those based on non-biodegradable pDMAEMA (poly(2-dimethylamino)ethyl methacrylate) and PEI (polyethylenimine) and with the regularly used lipidic transfection agent Lipofectamine. To promote endosomal escape, either the endosomolytic peptide diINF-7 was added to the formulations or photochemical internalization (PCI) was applied. Incubation of H1299 human lung cancer cells expressing firefly luciferase with polyplexes based on pHPMA-MPPM and TMC showed 30-40% silencing efficiency. This silencing activity was equal to or better than that obtained with the standard transfectants. Under all experimental conditions tested, the cytotoxicity of the biodegradable polymers was low. The application of PCI, as well as the addition of the diINF-7 peptide to the formulations increased their silencing activity up to 70-80%. This demonstrates that pHPMA-MPPM- and TMC-based polyplexes benefit substantially from endosomal escape enhancement. Importantly, the polyplexes retained their silencing activity in the presence of serum, and they showed low cytotoxicity. These biodegradable vectors are therefore attractive systems for further in vivo evaluations.

• Authors: Amir K Varkouhi, Twan Lammers, Raymond M Schiffelers, Mies J van Steenbergen, Wim E Hennink, Gert Storm
• Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (Eur J Pharm Biopharm) Vol. 77 Issue 3 Pg. 450-7 (Apr 2011) ISSN: 1873-3441 [Electronic] Netherlands
• PMID: 21118719 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2010 Elsevier B.V. All rights reserved.

Chemical References

• Acrylic Resins
• Biocompatible Materials
• Drug Carriers
• N-trimethyl chitosan chloride
• RNA, Small Interfering
• poly((2-hydroxypropyl) methacrylamide 1-methyl-2-piperidine methanol)
• Chitosan

Topics

• Acrylic Resins (chemistry)
• Biocompatible Materials (chemistry)
• Cell Line, Tumor
• Cell Survival (genetics)
• Chitosan (chemistry)
• Drug Carriers (chemistry)
• Electrophoresis, Agar Gel
• Gene Silencing (drug effects)
• Humans
• Microscopy, Confocal
• Molecular Structure
• RNA, Small Interfering (administration & dosage, genetics)
• Surface Properties
• Transfection