Firefly Luciferases

01/01/2018 - "In an in vivo AML model, NSG mice engrafted with MOLM-14 cells stably expressing firefly luciferase, both CAR33VH and CARMy96 efficiently eliminated tumors. "
07/01/2013 - "The aims of this study were to construct a tumor-specific bioluminescent eukaryotic vector driven by the hTERT gene promoter and to establish a stable HeLa cell line expressing a modified firefly luciferase gene. "
02/01/2010 - "Several imaging studies have used bioluminescent cancer cell lines expressing firefly luciferase to image tumor growth in live mice. "
10/21/2023 - "Lentiviral and adenoviral vectors expressing firefly luciferase transduced NSCLC tumors in vivo. "
01/01/2023 - "Human HT-29 cancer cells stably expressing marker genes GFP and firefly luciferase (GFP/luc) were cultured on human peritoneum and infected with two prototypic oncolytic viruses (GLV-0b347 and MeV-DsRed). "

09/01/2017 - "The glioma cell lines were genetically engineered to express firefly luciferase. "
01/01/2017 - "We used lentivirus to transduce the glioma cell line U251-MG with a tandem NF-κB reporter construct containing GFP and firefly luciferase allowing an assessment of NF-κB activity via fluorescence microscopy, flow cytometry, and luminometry. "
03/01/2014 - "U87 malignant glioma cells (U87-MG) stably transfected with firefly luciferase (Fluc) were implanted into BALB/cAnN nude mice by stereotactic injection into the striatum. "
10/01/2012 - "Growth of the firefly luciferase-labeled glioma cells was monitored through noninvasive biophotonic imaging. "
01/01/2012 - "Firefly luciferase (Fluc)-labeled human GBM8401 glioma cells were implanted into NOD-scid mice. "

04/01/2007 - "In the present study, we employed a CCD (charge-coupled-device) imaging system to monitor how a prostate-specific adenovirus vector (AdPSA-Luc) mediated the long-term, sustained expression of firefly luciferase (Luc) in living human prostate cancer mouse models. "
01/01/2011 - "In this chapter, we describe the use of firefly luciferase gene-based reporters and transfection protocols for the measurement of AR activity in heterologous COS-1 cells cotransfected with an AR expression vector and in VCaP prostate cancer cells expressing endogenous AR. "
04/01/2007 - "Non-invasive imaging of firefly luciferase reporter gene expression using bioluminescence imaging in human prostate cancer models."
03/01/2002 - "In this system, we found that firefly luciferase expression in prostate cancer cells can be varied over an 800-fold range. "
12/30/2000 - "The transcriptional activity of the 5'-flanking region was analyzed by transient transfection of rat prostate cancer cells with firefly luciferase-encoding gene expression vector constructs. "

01/01/2016 - "By applying immunohistochemistry and employing conditional-reporter mice that express firefly luciferase under the control of the IFN-β promoter in either all or only distinct cell types, we found that astrocytes are the main producers of IFN-β after infection of the brain with diverse neurotropic viruses, including rabies virus, Theiler's murine encephalomyelitis virus, and vesicular stomatitis virus. "
01/01/2022 - "Entry of recombinant vesicular stomatitis SARS-CoV-2 (VSVppSARS-2S) pseudovirus, having firefly luciferase and EGFP reporters, was assessed through luciferase assay and fluorescent microscopy. "
04/01/1996 - "Here we show that a human immunodeficiency virus type 1 (HIV-1)-based retroviral vector containing the firefly luciferase reporter gene can be pseudotyped with a broad-host-range vesicular stomatitis virus envelope glycoprotein G (VSV-G). "
01/01/2011 - "In this study, an improved IFN assay is presented which is based on a recombinant vesicular stomatitis virus (VSV) replicon encoding two reporter proteins, firefly luciferase and green fluorescent protein. "
04/08/2011 - "Armstrong 53b (Arm53b); baculovirus Autographa californica GP64 (GP64); charge-coupled device (CCD); dystroglycan (DG); feline immunodeficiency virus (FIV); glycoprotein precursor (GP-C); firefly luciferase (Luc); lymphocytic choriomeningitis virus (LCMV); nuclear targeted β-galactosidase (ntLacZ); optical density (OD); PBS/0.1% (w/v) Tween-20 (PBST); relative light units (RLU); Rous sarcoma virus (RSV); transducing units per milliliter (TU/ml); vesicular stomatitis virus (VSV-G); wheat germ agglutinin (WGA); 50% reduction in binding (C50)."

10/01/2010 - "We expressed firefly luciferase in CCR2b-expressing ATCs and observed improved homing (>10-fold) to CCL2-secreting neuroblastoma compared with CCR2-negative ATCs. "
09/01/2015 - "Human IMR-32 neuroblastoma cells, transfected with an ARE-firefly luciferase reporter, were exposed to extracts for 5 h. "
08/01/2013 - "We validated the use of a reporter containing the oxygen-dependent degradation domain of HIF-1α fused to firefly luciferase (ODD-luc) to monitor quantitatively distinct biochemical events leading to hypoxic HIF-1α expression or stabilization in a human neuroblastoma cell line (SH-SY5Y). "
01/01/1999 - "N2A mouse neuroblastoma cells, which express voltage-dependent sodium channels, were stably transfected with the reporter gene c-fos-luc, which contains the firefly luciferase gene under the transcriptional regulation of the human c-fos response element. "
07/01/2007 - "Neuroblastoma cells engineered to express the enzyme firefly luciferase were used to establish localized tumors and disseminated disease in SCID mice. "