Abstract | BACKGROUND AND OBJECTIVE: Current therapies for peri-implantitis apply the same clinical protocols as those used for the treatment of periodontitis; however, outcomes remain unpredictable. We hypothesized that resident fibroblasts of the peri-implantitis stroma and periodontitis stroma differ in their phenotype and response to host immune factors. Fibroblasts are highly heterogeneous and comprise discrete subtypes with the potential of modulating inflammatory activities. The aim of the present study was to characterize the expression of receptors for complement C1q of innate immunity on human peri-implantitis fibroblasts and investigate effects of C1q on the proinflammatory properties of the cells. MATERIAL AND METHODS: Fibroblasts were cultured from gingival tissues exhibiting peri-implantitis and periodontitis, and from healthy gingivae as a control. Expression of C1q receptors for the collagen (cC1qR) and globular domains (gC1qR) of the protein was determined by flow cytofluorometric analysis ( FITC) of specific antibodies bound to the surface of the cells. Secretion of C1q-inducible proinflammatory mediators was quantified after 24 h incubation using array-based ELISAs. RESULTS: CONCLUSIONS:
Peri-implantitis fibroblasts differ from periodontitis fibroblasts in phenotypic expression of cC1qR and function, and from healthy fibroblasts in proinflammatory, angiogenic and fibrogenic function. Peri-implantitis fibroblasts may represent a novel subtype.
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Authors | S Verardi, M Quaranta, S Bordin |
Journal | Journal of periodontal research
(J Periodontal Res)
Vol. 46
Issue 1
Pg. 134-40
(Feb 2011)
ISSN: 1600-0765 [Electronic] United States |
PMID | 21108646
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2010 John Wiley & Sons A/S. |
Chemical References |
- Chemokine CCL2
- Inflammation Mediators
- Interleukin-6
- Interleukin-8
- Membrane Glycoproteins
- Receptors, Complement
- Transforming Growth Factor beta1
- Vascular Endothelial Growth Factor A
- complement 1q receptor
- Complement C1q
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Topics |
- Cells, Cultured
- Chemokine CCL2
(biosynthesis)
- Complement C1q
(genetics)
- Fibroblasts
(immunology, metabolism)
- Fluorescent Antibody Technique
- Gingiva
(immunology, metabolism)
- Humans
- Immunity, Innate
- Immunophenotyping
- Inflammation Mediators
(immunology)
- Interleukin-6
(biosynthesis)
- Interleukin-8
(biosynthesis)
- Membrane Glycoproteins
(biosynthesis, genetics)
- Peri-Implantitis
(immunology, metabolism)
- Periodontitis
(immunology, metabolism)
- Protein Structure, Tertiary
- Receptors, Complement
(biosynthesis, genetics)
- Transforming Growth Factor beta1
(biosynthesis)
- Vascular Endothelial Growth Factor A
(biosynthesis)
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