Abstract | BACKGROUND: METHODS: Fifteen cases of PSTT, as well as samples from placental site nodule (PSN) (n = 2), leiomyosarcoma (n = 1), leiomyoma (n = 1), invasive cervical squamous cell carcinoma (n = 7) and endometrial adenocarcinoma (n = 11) were examined. Immunoreactivity was semi-quantitatively evaluated as negative (0, < 5% of cells stained), focally positive (1+, 5-10% of cells stained), positive (2+, 11-50% of cells stained) or diffusely positive (3+, > 50% of cells stained). Staining intensity for each subtype was graded from 0 to 3 and a mean intensity was calculated. RESULTS: Eighty percent of PSTT (12/15) were immunoreactive for GPC3 (0, 20; 1+, 20%; 2+, 40%; 3+, 20%) with a mean intensity of 1.3. Stronger, predominately cytoplasmic staining was seen in larger multi- and mononucleated cells with smaller mononucleate cells showing weak muddy cytoplasmic staining. Both PSN cases were positive (1+, 50%; 2+, 50%) and two of nine invasive cervical squamous cell carcinomas showed staining (0, 57%; 1+, 29%; 2+, 14%), predominately in a basal distribution. Other uterine tumors and non-neoplastic tissues were negative. CONCLUSIONS: Identification of GPC3 in PSTT and PSN is consistent with the derivation of these lesions from intermediate trophoblasts, which have been described to express GPC3. GPC3 may be a useful adjunct immunohistochemical marker in differentiating PSTT from non- trophoblastic tumors.
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Authors | Robin J Ou-Yang, Pei Hui, Ximing J Yang, Debra L Zynger |
Journal | Diagnostic pathology
(Diagn Pathol)
Vol. 5
Pg. 64
(Sep 25 2010)
ISSN: 1746-1596 [Electronic] England |
PMID | 20868507
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- GPC3 protein, human
- Glypicans
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Topics |
- Biomarkers, Tumor
(analysis)
- Female
- Glypicans
(analysis)
- Humans
- Immunohistochemistry
- Predictive Value of Tests
- Pregnancy
- Prognosis
- Trophoblastic Tumor, Placental Site
(chemistry, pathology)
- Uterine Neoplasms
(chemistry, pathology)
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