Anticancer effect of photodynamic therapy with hexenyl ester of 5-aminolevulinic acid in oral squamous cell carcinoma.

Abstract

BACKGROUND:

Five-aminolaevulinic acid (ALA) and its derivatives act as precursors of the photosensitizer protoporphyrin IX (PpIX). In this study, the effect of photodynamic therapy (PDT) with hexenyl ester of ALA (ALA-hx) was examined in a human oral squamous cell carcinoma, YD10B cells.

METHODS:

PpIX accumulation and mRNA expression of coproporphyrinogen oxidase (CPO) by ALA and ALA-hx was examined. Cell viability was examined by MTT assay and the molecular mechanism was investigated.

RESULTS:

The PpIX synthesis and mRNA expression of CPO was much higher in the cells treated with ALA-hx than ALA. At the concentration that PDT with ALA did not affect cell growth, ALA-hx PDT effectively produced reactive oxygen species (ROS) and suppressed cell growth. Growth inhibition by ALA-hx PDT was due to mitochondrial-dependent apoptosis.

CONCLUSION:

Our results suggest that ALA-hx PDT effectively induced apoptosis of YD-10B cells and can be considered as a therapeutic alternative for oral cancer.

• Authors: Yeon-Hee Moon, Jong-Hwan Park, Soo-A Kim, Jee-Bum Lee, Sang-Gun Ahn, Jung-Hoon Yoon
• Journal: Head & neck (Head Neck) Vol. 32 Issue 9 Pg. 1136-42 (Sep 2010) ISSN: 1097-0347 [Electronic] United States
• PMID: 19953630 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protoporphyrins
• RNA, Messenger
• Reactive Oxygen Species
• Aminolevulinic Acid
• protoporphyrin IX
• Caspase 3

Topics

• Aminolevulinic Acid (pharmacology)
• Blotting, Western
• Carcinoma, Squamous Cell (drug therapy)
• Caspase 3 (drug effects, metabolism)
• Cell Death (drug effects)
• Cell Survival (drug effects)
• Flow Cytometry
• Humans
• Mouth Neoplasms (drug therapy)
• Photochemotherapy (methods)
• Protoporphyrins (metabolism)
• RNA, Messenger (analysis)
• Reactive Oxygen Species (metabolism)
• Reference Values
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured