Peroxisome proliferator-activated receptor-gamma agonists suppress iNOS expression induced by LPS in rat primary Schwann cells.

Abstract

In bacterial-induced peripheral nervous system (PNS) inflammation, Schwann cells (SCs) are activated, producing inducible nitric oxide synthase (iNOS), contributed to the pathogenesis of demyelinating disease, such as multiple sclerosis. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) has been shown to play a protective role in cellular inflammatory responses. Here we showed that LPS-induced iNOS biosynthesis was in a concentration and time-dependent manner. In LPS-treated primary SCs, retreatment with PPAR-gamma agonist remitted the increase of iNOS, p38 phosphorylation and TLR4, MyD88, augmented the expression of PPAR-gamma and localization in nuclear. Coadministration of GW 9662 reversed the effect of PPAR-gamma agonists. These results suggest that PPAR-gamma agonists, 15d-PGJ(2) and pioglitazone, had the anti-inflammatory effects.

• Authors: Fupeng Zhang, Fen Liu, Meijuan Yan, Huoyan Ji, Ling Hu, Xiaohong Li, Ji Qian, Xingxin He, Li Zhang, Aiguo Shen, Chun Cheng
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 218 Issue 1-2 Pg. 36-47 (Jan 25 2010) ISSN: 1872-8421 [Electronic] Netherlands
• PMID: 19942298 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 Elsevier B.V. All rights reserved.

Chemical References

• 15-deoxyprostaglandin J2
• 2-chloro-5-nitrobenzanilide
• Anilides
• Anti-Inflammatory Agents
• Enzyme Inhibitors
• Lipopolysaccharides
• Myd88 protein, rat
• Myeloid Differentiation Factor 88
• PPAR gamma
• Thiazolidinediones
• Tlr4 protein, rat
• Toll-Like Receptor 4
• Nitric Oxide Synthase Type II
• p38 Mitogen-Activated Protein Kinases
• Prostaglandin D2
• Pioglitazone

Topics

• Anilides (pharmacology)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Blotting, Western
• Cell Line
• Down-Regulation
• Enzyme Inhibitors (pharmacology)
• Fluorescent Antibody Technique
• Lipopolysaccharides (pharmacology)
• Myeloid Differentiation Factor 88 (biosynthesis, drug effects)
• Nitric Oxide Synthase Type II (biosynthesis, drug effects)
• PPAR gamma (agonists, drug effects)
• Pioglitazone
• Prostaglandin D2 (analogs & derivatives, pharmacology)
• Rats
• Rats, Sprague-Dawley
• Reverse Transcriptase Polymerase Chain Reaction
• Schwann Cells (metabolism)
• Signal Transduction (drug effects, physiology)
• Thiazolidinediones (pharmacology)
• Toll-Like Receptor 4 (biosynthesis, drug effects)
• p38 Mitogen-Activated Protein Kinases (drug effects, metabolism)