[Effect of GnRH-II on VEGF secreted by stromal cells from endometrium of endometriosis].

Abstract

OBJECTIVE:

To inspect the effect of gonadotropin-releasing hormonel II (GnRH-II)on the secretion of VEGF by eutopic and ectopic endometrial stromal cells cultured in vitro.

METHODS:

Eutopic and ectopic stromal cells cultured in vitro were treated with different concentrations (1x10(-10) - 1x10(-6) mol/L) of GnRH-II,and the control group was not treated by GnRH-II.Enzyme-linked immunosorbent assay (ELISA) was used to measure the VEGF protein in the medium of the above 2 groups.

RESULTS:

There was no difference between the VEGF protein expressed by eutopic and ectopic stromal cells in the medium after culturing in vitro for 48 hours (P>0.05). The 1x10(-10) - 1x10(-6) mol/L GnRH-II could dose-dependently reduce VEGF protein secreted by endometrial stromal cells (P<0.01), and the inhibition to ectopic endometrial stromal cells was stronger than that to eutopic endometrial stromal cells (P<0.01).

CONCLUSION:

Ectopic stromal cells cultured in vitro can secrete VEGF, and so can eutopic stromal cells. This may play an important role in the formation and development of endometriosis. GnRH-II can reduce VEGF protein secreted by ectopic endometrial stromal cells cultured in vitro, and its inhibition is stronger than that of eutopic endometrial stromal cells.

• Authors: Qiuhong Liu, Fengying Huang, Huanping Wang, Ying Zou
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 34 Issue 9 Pg. 926-32 (Sep 2009) ISSN: 1672-7347 [Print] China
• PMID: 19779269 (Publication Type: Journal Article)

Chemical References

• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• Gonadotropin-Releasing Hormone
• LHRH, His(5)-Trp(7)-Tyr(8)-

Topics

• Adult
• Cells, Cultured
• Endometriosis (metabolism, pathology)
• Endometrium (metabolism, pathology)
• Female
• Gonadotropin-Releasing Hormone (analogs & derivatives, pharmacology)
• Humans
• Middle Aged
• Stromal Cells (cytology, metabolism)
• Vascular Endothelial Growth Factor A (metabolism)
• Young Adult