Abstract | PURPOSE: METHODS: RESULTS: Expression of NG-2 was robust in C, CT, DT, and mild in D. The intensity of IB-4 was higher in D and DT compared with the C and CT. Ox-42 and ED-1 expression was higher in the D than in the DT, C or CT. Expression of VEGF and HO-1 was non-specific across the four groups. Plasma levels of 15-F-2t-IsoP and TNF-alpha were higher in the D as compared with the C, CT and DT. SOD levels were lower in the D when compared with the C, CT and D. CONCLUSIONS: Macrophage/microglia activation, pericyte loss and endothelial/perivascular cell changes occur early in the pathogenesis of DR. These changes are associated with an increase in plasma markers of oxidative stress and inflammation and are minimized by treatment with NAC. The results suggest that therapies that reduce free radicals will help minimize the early events in diabetic retinopathy in the STZ model.
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Authors | Gina Y Tsai, Jing Z Cui, Husnain Syed, Zhengyuan Xia, Ugur Ozerdem, John H McNeill, Joanne A Matsubara |
Journal | Clinical & experimental ophthalmology
(Clin Exp Ophthalmol)
Vol. 37
Issue 2
Pg. 223-31
(Mar 2009)
ISSN: 1442-9071 [Electronic] Australia |
PMID | 19723131
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Free Radical Scavengers
- Isoprostanes
- Tumor Necrosis Factor-alpha
- 8-epi-prostaglandin F2alpha
- Dinoprost
- Superoxide Dismutase
- Acetylcysteine
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Topics |
- Acetylcysteine
(therapeutic use)
- Animals
- Biomarkers
(blood)
- Diabetes Mellitus, Experimental
(drug therapy, metabolism, pathology)
- Diabetic Retinopathy
(drug therapy, metabolism, pathology)
- Dinoprost
(analogs & derivatives)
- Endothelium, Vascular
(metabolism, pathology)
- Enzyme-Linked Immunosorbent Assay
- Fluorescent Antibody Technique, Indirect
- Free Radical Scavengers
(therapeutic use)
- Immunoenzyme Techniques
- Inflammation
(metabolism, pathology)
- Isoprostanes
(blood)
- Macrophages
(metabolism, pathology)
- Male
- Microglia
(metabolism, pathology)
- Oxidative Stress
- Pericytes
(metabolism, pathology)
- Rats
- Rats, Wistar
- Retina
(drug effects, metabolism)
- Superoxide Dismutase
(blood)
- Tumor Necrosis Factor-alpha
(blood)
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