Abstract | BACKGROUND: METHODS: We determined HPSE expression by Western blot analysis in ARMS and ERMS cells lines and activity in supernatants by an ELISA assay. Stable HPSE silencing has been performed by shRNA technique in RH30 and RD cell lines and their invasiveness has been evaluated by Matrigel-invasion assay. HPSE activity and mRNA expression have also been quantified in plasma and biopsies from RMS patients. RESULTS: HPSE expression and activity have been detected in all RMS cell lines. Stable HPSE silencing by shRNA technique determined a significant knockdown of gene expression equal to 76% and 58% in RH30 and RD cell lines respectively and induced a less invasive behaviour compared to untreated cells. Finally, we observed that HPSE mRNA expression in biopsies was higher than in foetal skeletal muscle and that plasma from RMS patients displayed significantly more elevated HPSE levels than healthy subjects with a trend to higher levels in ARMS. CONCLUSION: In conclusion, our data demonstrate for the first time HPSE expression and activity in RMS and highlight its involvement in tumor cell invasion as revealed by shRNA silencing. Moreover, HPSE expression in RMS patients is significantly higher with respect to healthy subjects. Further studies are warranted to assess possible relationships between HPSE and clinical behaviour in RMS.
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Authors | Valentina Masola, Claudio Maran, Evelyne Tassone, Angelica Zin, Angelo Rosolen, Maurizio Onisto |
Journal | BMC cancer
(BMC Cancer)
Vol. 9
Pg. 304
(Aug 28 2009)
ISSN: 1471-2407 [Electronic] England |
PMID | 19715595
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adolescent
- Case-Control Studies
- Cell Line, Tumor
- Child
- Child, Preschool
- Cohort Studies
- Female
- Gene Expression Regulation, Neoplastic
- Glucuronidase
(genetics, metabolism)
- Humans
- Infant
- Male
- Neoplasm Invasiveness
- Rhabdomyosarcoma, Alveolar
(embryology, enzymology, pathology)
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