Gene and protein expression of cartilage oligomeric matrix protein associated with oncogenesis in canine tumors.

Abstract

To investigate the expression of cartilage oligomeric matrix protein (COMP) associated with oncogenesis, samples of serum and tumor tissue were obtained from 25 dogs with tumors. The serum levels of COMP in dogs with tumors were significantly higher than in 38 normal controls and correlated with the concentrations of tumor tissue extracts. Positive bands to COMP antibody were detected on immunoblots of tumor extracts. Immunohistochemistry and in situ hybridization demonstrated positive signals of COMP and its mRNA in the cytoplasm of tumor cells from mammary gland tumors, but not in the normal mammary gland. Positive immunohistochemistry results were also obtained for COMP in mast cell tumor and melanoma cells. Oncogenesis might augment production of COMP and the serum level of COMP in dogs.

• Authors: Kazuto Yamanokuchi, Akira Yabuki, Yuki Yoshimoto, Ko Arai, Makoto Fujiki, Kazuhiro Misumi
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 71 Issue 4 Pg. 499-503 (Apr 2009) ISSN: 0916-7250 [Print] Japan
• PMID: 19420857 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Extracellular Matrix Proteins
• Glycoproteins
• Matrilin Proteins

Topics

• Animals
• Dog Diseases (blood, genetics, metabolism)
• Dogs
• Enzyme-Linked Immunosorbent Assay (veterinary)
• Extracellular Matrix Proteins (biosynthesis, blood, genetics, metabolism)
• Female
• Glycoproteins (biosynthesis, blood, genetics, metabolism)
• Immunohistochemistry (veterinary)
• In Situ Hybridization (veterinary)
• Male
• Matrilin Proteins
• Neoplasms (blood, genetics, metabolism, veterinary)