Abstract |
Lower numbers of Vgamma9Vdelta2 T cells in cord blood (CB) than in adult peripheral blood (PB), as well as their impaired ability to produce interferon-gamma (IFN-gamma) in response to stimulation, are associated with functional deficiency in the immune system in newborns. In this study, we stimulated CB Vgamma9 T cells with their T-cell receptor-specific ligand, isopentenyl pyrophosphate ( IPP), plus exogenous costimulatory cytokines such as interleukin-2 (IL-2), IL-12 and tumour necrosis factor-alpha ( TNF-alpha), which are known to play important roles in the activation of PB gammadelta T cells. Our data show that CB Vgamma9 T cells are able to produce IFN-gamma at levels comparable to PB Vgamma9 T cells by the addition of TNF-alpha in the presence of IPP and IL-2; however, under the same culture conditions, IL-12 does not efficiently activate CB Vgamma9 T cells to produce IFN-gamma. The frequency of TNF-alpha receptor II-positive Vgamma9T cells and the expression levels of TNF-alpha receptor II are similar in CB and PB; in contrast, the frequency of IL-12 receptor betaI (IL-12RbetaI) -positive Vgamma9T cells and expression levels of IL-12RbetaI are significantly lower in CB than PB. TNF-alpha but not IL-12 increases the expression of IL-2Rbeta on CB Vgamma9 T cells. These results provide new insights into the role of TNF-alpha in the activation of CB Vgamma9 T cells.
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Authors | Eduardo Jose Campos Alberto, Naoki Shimojo, Masahiko Aoyagi, Yoichi Kohno |
Journal | Immunology
(Immunology)
Vol. 127
Issue 2
Pg. 171-7
(Jun 2009)
ISSN: 1365-2567 [Electronic] England |
PMID | 19019091
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Hemiterpenes
- Organophosphorus Compounds
- Receptors, Antigen, T-Cell, gamma-delta
- Tumor Necrosis Factor-alpha
- Interleukin-12
- isopentenyl pyrophosphate
- Interferon-gamma
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Topics |
- Adult
- Cells, Cultured
- Fetal Blood
(immunology)
- Hemiterpenes
(immunology)
- Humans
- Infant, Newborn
- Interferon-gamma
(biosynthesis)
- Interleukin-12
(immunology)
- Lymphocyte Activation
(immunology)
- Middle Aged
- Organophosphorus Compounds
(immunology)
- Receptors, Antigen, T-Cell, gamma-delta
(blood)
- T-Lymphocyte Subsets
(immunology)
- Tumor Necrosis Factor-alpha
(immunology)
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