Albumin resuscitation protects against traumatic/hemorrhagic shock-induced lung apoptosis in rats.

Abstract

OBJECTIVE:

To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock (T/HS) rats.

METHODS:

Studies were performed on an in vivo model of spontaneously breathing rats with induced T/HS; the rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 min with Ringer's lactate solution (RS) or 5% (w/v) albumin (ALB), and the left lower lobes of the lungs were resected.

RESULTS:

Albumin administered during reperfusion markedly attenuated injury of the lung and decreased the concentration of lactic acid and the number of in situ TdT-mediated dUTP nick-end labelling (TUNEL)-positive cells. Moreover, immunohistochemistry performed 24 h after reperfusion revealed increases in the level of nuclear factor kappaB (NF-kappaB), and phosphorylated p38 mitogen-activated protein kinase (MAPK) in the albumin-untreated group was down-regulated by albumin treatment when compared with the sham rats.

CONCLUSION:

Resuscitation with albumin attenuates tissue injury and inhibits T/HS-induced apoptosis in the lung via the p38 MAPK signal transduction pathway that functions to stimulate the activation of NF-kappaB.

• Authors: Yun Zhang, Zhong-Yan Liang, Shao-Yang Zhang, Fang-Fang Huang, Wei Wu, Yuan Gao, Zuo-Bing Chen
• Journal: Journal of Zhejiang University. Science. B (J Zhejiang Univ Sci B) Vol. 9 Issue 11 Pg. 871-8 (Nov 2008) ISSN: 1673-1581 [Print] China
• PMID: 18988306 (Publication Type: Journal Article)

Chemical References

• Albumins
• Transcription Factor RelA
• Lactic Acid
• p38 Mitogen-Activated Protein Kinases
• Oxygen

Topics

• Albumins (administration & dosage)
• Animals
• Apoptosis (drug effects)
• Enzyme Activation
• Immunohistochemistry
• In Situ Nick-End Labeling
• Lactic Acid (blood)
• Lung Diseases (metabolism, pathology, prevention & control)
• Male
• Oxygen (blood)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy, metabolism, pathology)
• Resuscitation (methods)
• Shock, Hemorrhagic (drug therapy, metabolism, pathology)
• Transcription Factor RelA (metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)