Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis.

Abstract

INTRODUCTION:

MicroRNAs (miRNAs) are small (approximately 22 nucleotides) regulatory RNAs which play fundamental roles in many human diseases, including cancer. There is no report on the miRNA expression profile of retinoblastoma.

METHODS:

This work was undertaken to identify differentially expressed miRNAs in human retinoblastoma tissues by microRNA microarray technique, and some miRNAs were verified using northern blot analysis and the in situ hybridization method.

RESULTS:

A cluster of microRNAs was identified as highly expressed in retinoblastoma, including hsa-miR-494, hsa-let-7e, hsa-miR-513-1, hsa-miR-513-2, hsa-miR-518c*, hsa-miR-129-1, hsa-miR-129-2, hsa-miR-198, hsa-miR-492, hsa-miR-498, hsa-miR-320, hsa-miR-503, and hsa-miR-373*.

CONCLUSION:

These miRNAs are the first to be reported for human retinoblastoma and may play significant roles in regulating tumor genesis.

• Authors: Jian-Jun Zhao, Juhua Yang, Jianhong Lin, Nan Yao, Yihua Zhu, Jianlong Zheng, Jianhua Xu, Jin Q Cheng, Jian-Yin Lin, Xu Ma
• Journal: Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery (Childs Nerv Syst) Vol. 25 Issue 1 Pg. 13-20 (Jan 2009) ISSN: 1433-0350 [Electronic] Germany
• PMID: 18818933 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MIRN373 microRNA, human
• MicroRNAs
• mirnlet7 microRNA, human

Topics

• Adult
• Blotting, Northern
• Cluster Analysis
• Female
• Gene Expression Profiling (methods)
• Gene Expression Regulation, Neoplastic
• Humans
• In Situ Hybridization
• Male
• MicroRNAs (genetics)
• Oligonucleotide Array Sequence Analysis (methods)
• Retinoblastoma (genetics)