Abstract | BACKGROUND: The cockroach is a major source of indoor allergens, which cause perennial rhinitis and asthma. Per a 7 is one of the major allergens from the American cockroach, eliciting IgE reaction in over 50% of sera from allergic individuals. However, the actions of Per a 7 in the pathogenesis of allergic diseases remain poorly understood. OBJECTIVE: The aim of the study was to investigate the effects of Per a 7 on the expression of protease-activated receptors (PARs) on murine mast cells and the release of T-helper type 2 (Th2) cytokines from these cells. Methods Per a 7 gene was cloned and expressed in Escherichia coli. The purified recombinant Per a 7 (rPer a 7) was used to challenge murine mast cells P815 and the expression of PARs was determined by using real-time quantitative RT-PCR and flow cytometry. The release of IL-4 and IL-13 was detected with ELISA. RESULTS: rPer a 7 was solubly expressed in E. coli and the purified rPer a 7 reacted with 75% (six out of eight) of the sera from cockroach-allergic patients. Following stimulation of rPer a 7, the expression of PAR-1, PAR-2, PAR-3 and PAR-4 mRNAs on P815 cells increased by 8.7-, 6.8-, 14.4- and 8.8-fold, respectively, following 2- and 6-h incubation periods, and the expression of PAR-1, PAR-2 and PAR-4 proteins was enhanced following 16-h incubation. rPer a 7 provoked approximately up to a 4.8- and 2.3-fold increase in IL-4 and IL-13 release from P815 cells following 6- and 16-h incubation periods. CONCLUSION: rPer a 7 induced up-regulation of PAR expression on P815 cells and provoked Th2 cytokines IL-4 and IL-13 secretion from these murine mast cells, which implicated that this major cockroach allergen is likely to contribute to the development of cockroach-related allergic disease.
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Authors | Z Zhang, H Zhang, H Yang, L Zhang, X Chen, X Zheng, S He |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 38
Issue 7
Pg. 1160-7
(Jul 2008)
ISSN: 1365-2222 [Electronic] England |
PMID | 18691294
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Allergens
- Antigens, Plant
- Interleukin-13
- Receptors, Proteinase-Activated
- Recombinant Proteins
- allergen Per a 7, Periplaneta americana
- Interleukin-4
- Immunoglobulin E
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Topics |
- Allergens
(immunology)
- Animals
- Antigens, Plant
- Cell Line, Tumor
- Cockroaches
(immunology)
- Gene Expression
- Humans
- Immunoglobulin E
(blood)
- Interleukin-13
(biosynthesis, immunology)
- Interleukin-4
(biosynthesis, immunology)
- Mast Cells
(immunology, metabolism)
- Mice
- Receptors, Proteinase-Activated
(metabolism)
- Recombinant Proteins
(immunology)
- Th2 Cells
(immunology, metabolism)
- Up-Regulation
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